Searching for a "Hidden" Prophage in a Marine Bacterium

CORC > 厦门大学 > 海洋环境－已发表论文

	Searching for a "Hidden" Prophage in a Marine Bacterium
	Zhao, Y. L. ; Wang, K. ; Ackermann, H. W. ; Halden, R. U. ; Jiao, N. Z. ; Chen, F. ; Jiao NZ(焦念志)
刊名	http://dx.doi.org/10.1128/AEM.01450-09
	2010-01-15
关键词	GENE-TRANSFER AGENT CHESAPEAKE BAY LYSOGENY INDUCTION VIRUSES PHAGE COMMUNITIES GENOME BACTERIOPLANKTON IDENTIFICATION
英文摘要	National Science Foundation [MCB-0132070, MCB-0238515, MCB-0537041]; Xiamen University [2007CB815904]; Prophages are common in many bacterial genomes. Distinguishing putatively viable prophages from nonviable sequences can be a challenge, since some prophages are remnants of once-functional prophages that have been rendered inactive by mutational changes. In some cases, a putative prophage may be missed due to the lack of recognizable prophage loci. The genome of a marine roseobacter, Roseovarius nubinhibens ISM (hereinafter referred to as ISM), was recently sequenced and was reported to contain no intact prophage based on customary bioinformatic analysis. However, prophage induction experiments performed with this organism led to a different conclusion. In the laboratory, virus-like particles in the ISM culture increased more than 3 orders of magnitude following induction with mitomycin C. After careful examination of the ISM genome sequence, a putative prophage (ISM-pro1) was identified. Although this prophage contains only minimal phage-like genes, we demonstrated that this "hidden" prophage is inducible. Genomic analysis and reannotation showed that most of the ISM-pro1 open reading frames (ORFs) display the highest sequence similarity with Rhodobacterales bacterial genes and some ORFs are only distantly related to genes of other known phages or prophages. Comparative genomic analyses indicated that ISM-pro1-like prophages or prophage remnants are also present in other Rhodobacterales genomes. In addition, the lysis of ISM by this previously unrecognized prophage appeared to increase the production of gene transfer agents (GTAs). Our study suggests that a combination of in silico genomic analyses and experimental laboratory work is needed to fully understand the lysogenic features of a given bacterium.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/60500]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Zhao, Y. L.,Wang, K.,Ackermann, H. W.,et al. Searching for a "Hidden" Prophage in a Marine Bacterium[J]. http://dx.doi.org/10.1128/AEM.01450-09,2010.
APA	Zhao, Y. L..,Wang, K..,Ackermann, H. W..,Halden, R. U..,Jiao, N. Z..,...&焦念志.(2010).Searching for a "Hidden" Prophage in a Marine Bacterium.http://dx.doi.org/10.1128/AEM.01450-09.
MLA	Zhao, Y. L.,et al."Searching for a "Hidden" Prophage in a Marine Bacterium".http://dx.doi.org/10.1128/AEM.01450-09 (2010).