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	肝豆灵干预Wilson病模型大鼠NMR代谢组学研究; Metabonomic Research on Curative Mechanism of Gandouling Treating Model Rats of Wilson's Disease
	蒋怀周 ; 危阳洋 ; 蒋昭雨 ; 董继扬 ; 蒋雨晨
	2014-09-05
关键词	Wilson病 代谢组学 核磁共振 尿液 小分子代谢物 肝豆灵 Wilson's disease metabonomics nuclear magnetic resonance urine small molecular metabolite Gandouling
英文摘要	目的:以基于核磁共振(nuClEAr MAgnETIC rESOnAnCE,nMr)的代谢组学方法对肝豆灵干预WIlSOn病(WIlSOn'S dISEASE,Wd)模型大鼠进行研究,分析肝豆灵干预后,Wd模型大鼠尿液中代谢物的变化,从小分子层面探讨肝豆灵对Wd的治疗作用机制。方法:42只雄性WISTAr大鼠,体重(180±20)g,随机被分为健康对照组(n=14)、模型组(n=14)和肝豆灵组(n=14)。采用铜负荷法制作WIlSOn病大鼠模型,以nMr技术对大鼠尿液进行检测,经MESTrE-C 2.3软件及自编软件对谱图进行手动调相、基线校正和谱峰对齐。对样品进行分段积分,将积分数据归一化后构成数据矩阵,并利用PCA方法对数据矩阵进行统计分析。结果:相对于模型组,肝豆灵组大鼠尿液色氨酸(TryPTOPHAn)、马尿酸盐(HIPPurATE)、苯基丙氨酸(PHEnylAlAnInE)、甘氨酸(glyCInE)、苯乙酰甘氨酸(PAg)含量有显著升高;乳酸(lACTATE)、甜菜碱(TMAO/bETAInE)、甲酸盐(fOrMATE)、二甲基甘氨酸(dMg)含量有所升高;柠檬酸盐(CITrATE)、肌氨酸酐(CrEATInInE)、谷氨酰胺(gluTAMInE)含量显著降低;丙酮酸盐(PyruVATE)甲基胍(METHylguAnIdInE)、丙酮(ACETOnE)、牛磺酸(TAurInE)含量有所降低。这些发生改变的代谢物可为进一步研究肝豆灵干预Wd的作用机制提供参考。; Objective:Applying 1H nuclear magnetic resonance(1H-NMR)based metabonomics to study the changes of small molecular metabolites in the urine of model rats of WD treated with Gandouling.To explore the curative mechanism of Gandouling on the model rats in small molecular aspect.Methods:42 male Wistar rats[weight=(180±20)g]were divided into three groups randomly,the model group(n=14),the control group(n=14),and the Gandouling group(n=14),with the models established with copper-loaded method.Urine of the rats was tested with 1H-NMR technology.The spectra were edited with MestRe-C2.3 and self-programmed software and then principal component analysis(PCA)was applied to differentiate the three groups.Results:Compared with those of the model group,tryptophan,hippurate,phenylalanine,glycine and PAG concentration were found to be significantly higher;lactate,TMAO/betaine,formate and DMG were higher;citrate,creatinine and glutamine were significantly lower;pyruvate,methylguanidine,acetone and taurine were lower in the urine of the Gandouling group.The small molecular metabolites mentioned above may contribute to the curative mechanism of Gandouling,and provide references for further researches on the treatment mechanism of WD.; 安徽省高校自然科学基金重点项目(KJ2012Z228); 安徽中医学院自然科学基金项目(2011ZR008B)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/124584]
专题	材料学院－已发表论文
推荐引用方式 GB/T 7714	蒋怀周,危阳洋,蒋昭雨,等. 肝豆灵干预Wilson病模型大鼠NMR代谢组学研究, Metabonomic Research on Curative Mechanism of Gandouling Treating Model Rats of Wilson's Disease[J],2014.
APA	蒋怀周,危阳洋,蒋昭雨,董继扬,&蒋雨晨.(2014).肝豆灵干预Wilson病模型大鼠NMR代谢组学研究..
MLA	蒋怀周,et al."肝豆灵干预Wilson病模型大鼠NMR代谢组学研究".(2014).

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