厦门文昌鱼(Branchiostoma belcheri Gray)种群杂合性研究

CORC > 集美大学

	厦门文昌鱼(Branchiostoma belcheri Gray)种群杂合性研究
	陈锦 ; 黎中宝 ; 赵斌丽 ; 雷光高 ; 张桂玲 ; 王展林
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hyfz200901015&dbcode=CJFQ&dbname=CJFQ2009
	2012-06-05 ; 2012-06-05
关键词	文昌鱼等位酶杂合子缺乏杂合子过量 Branchiostoma belcheri Allozyme Heterozygote deficiency Heterozygote excess Q953
其他题名	HETEROZYGOSITY OF FOUR POPULATINONS OF BRANCHIOSTOMA BELCHERI GRAY IN XIAMEN
中文摘要	应用聚丙烯凝胶电泳技术,研究了厦门海域四个野生文昌鱼种群的杂合度缺乏和过量情况。在测定的7个酶系统中,共有14个等位酶基因位点,分别为Aat-1、Amy-1、Amy-2、Amy-3、Amy-4、Me-1、Me-2、Mdh-1、Mdh-2、Sod-1、Sod-2、Est-1、Est-2、Per-1。多态位点有8个,分别为Sod-1、Aat-1、Me-1、Me-2、Mdh-1、Mdh-2、Est-2、Amy-2。结果表明,在大嶝岛种群、南线至十八线东端种群、南线至十八线西端种群及黄厝种群中,多态位点Sod-1、Mdh-2、Me-2、Aat-1显著偏离H-W平衡标准(P<0.05),而位点Me-1、Mdh-1、Est-2符合H-W平衡标准(P>0.05)。多态位点Amy-2除南线至十八线东端种群显著偏离H-W平衡标准(P<0.05)外,其它三个种群的多态位点Amy-2均符合H-W平衡标准(P>0.05)。在所有的8个多态位点中,只有大嶝岛和黄厝两个种群的Amy-2位点表现为杂合子缺失(F>0),其它种群的多态位点均表现为杂合子过量(F<0)。造成杂合体缺失的主要原因可能是自然选择、近交、哑等位基因等。杂合体的缺失会导致某些基因从该物种的基因库中消失,从而降低了物种的遗传多样性,同时也使物种对环境的适应能力降低。; Heterozygosity of Branchiostoma belcheri Gray in Xiamen was investigated using vertical slab polyacryla-mide gel electrophoresis.Seven enzymes presumably encoded by 14 allozyme loci in B.belcheri.They are Aat-1,Amy-1,Amy-2,Amy-3,Amy-4,Me-1,Me-2,Mdh-1,Mdh-2,Sod-1,Sod-2,Est-1,Est-2,and Per-1,of which 8 loci are polymorphic,they are Sod-1,Aat-1,Me-1,Me-2,Mdh-1,Mdh-2,Est-2,Amy-2.The genotypic distribution observed at Me-1,Mdh-1 and Est-2 in all populations and Amy-2 in East of Southern line to 18 population was in agreement with those expected by the Hardy-Weinberg equilibrium(HWE)(P>0.05);but Sod-1,Mdh-2,Me-2 and Aat-1 loci in all populations and Amy-2 in other three populations were significant departed from those expected from HWE(P<0.05).Heterozygote deficiency in Amy-2 locus in East of Southern line to 18 and Huangcuo populations(F>0) and heterozygote excess in other polymorphic loci(F<0) were also revealed among populations.The heterozygote deficiency resulting from natural selection,inbreeding effects,presence of null allele and so on would cause disappearance of allele,lower genetic diversity and lower environmental adaptation ability.; 【作者单位】集美大学水产学院；集美大学水产生物技术研究所；福建省高校水产科学技术与食品安全重点实验室；【作者英文名】CHEN Jin,LI Zhong-Bao,ZHAO Bin-Li,LEI Guang-Gao,ZHANG Gui-Ling,WANG Zhan-Lin(Fishery College,Jimei University,Xiamen,361021;Institute of Aquaculture Biotechnology,Jimei University,Xiamen,361021;Key Laboratory of Science and Technology for Aquaculture
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12623]
专题	集美大学
推荐引用方式 GB/T 7714	陈锦,黎中宝,赵斌丽,等. 厦门文昌鱼(Branchiostoma belcheri Gray)种群杂合性研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hyfz200901015&dbcode=CJFQ&dbname=CJFQ2009,2012, 2012.
APA	陈锦,黎中宝,赵斌丽,雷光高,张桂玲,&王展林.(2012).厦门文昌鱼(Branchiostoma belcheri Gray)种群杂合性研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hyfz200901015&dbcode=CJFQ&dbname=CJFQ2009.
MLA	陈锦,et al."厦门文昌鱼(Branchiostoma belcheri Gray)种群杂合性研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hyfz200901015&dbcode=CJFQ&dbname=CJFQ2009 (2012).