应用荧光抗体技术检测牙鲆体内的河流弧菌

CORC > 集美大学

	应用荧光抗体技术检测牙鲆体内的河流弧菌
	鄢庆枇 ; 邹文政 ; 纪荣兴 ; 庄峙厦 ; 王小如
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hykx200604003&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	荧光抗体检测技术牙鲆(Paralichthysolivaceus) 河流弧菌(Vibriofluvialis) fluorescent antibody technique Paralichthys oliwaceus Vibrio fluvialis S943
其他题名	Detection of Vibrio fluviails in Paralichthys olivaceus using indirect fluorescent antibody technique
中文摘要	用灭活的河流弧菌(Vibrio fluvialis)抗原,注射免疫实验兔,制得凝集价为1∶5 120的抗血清。用试管凝集法检测了抗血清的特异性,再用免疫吸附法去除交叉反应,从而得到高效价、高特异性的抗河流弧菌血清。用所制备的抗血清在实验室中建立起河流弧菌荧光抗体检测技术(FAT),在荧光显微镜下可清楚地看到被标记的病原菌,整个检测过程只需3h。用河流弧菌感染牙鲆(Paralichthys olivaceus),24 h后,用FAT测定牙鲆的血液、肾脏和肝脏中的河流弧菌,在血液中河流弧菌的检出率最高,其次是肾脏,肝脏的检出率最低。以上结果表明:荧光抗体技术可以快速、灵敏、准确地检测出牙鲆体内的河流弧菌。; Vibrio fluvialis,the pathogenic bacteria of Paralichthys oliwaceus were inactivated for vaccine.using an anti-V.fluvialis sera,agglutination titer up to 1∶5 120,was got from rabbits by injected vaccine.The serum has no cross reaction to Vibrio parahaemolyticus and other 11 trains after being absorbed.Using the anti sera,indirect fluorescent antibody technique for detection of V. fluvialis was established.The pathogenic bacteria could been observed clearly under fluorescent microscope.The whole process lasts merely 3 h.The P.oliwaceus was artificially infected by injecting of V.fluvialis,and after 24 h post injection,the blood,kidney and liver of P.oliwaceus were detected for V.fluvialis by indirect fluorescent antibody technique.The results showed that the ratio of V.fluvialis detected in blood was higher than those in kidney and liver.The results indicated that FAT would be used to detect V.fluvialis in P.olivaceus rapidly,sensitively and truly.; 【作者单位】集美大学水产学院；厦门大学化学化工学院；厦门大学化学化工学院福建厦门361021；福建厦门361005；福建厦门361021；【作者英文名】YAN Qing-pi~(1,2),ZOU Wen-zheng~(1),JI Rong-xing~(1),ZHUANG Zhi-xia~(2),WANG Xiao-ru~(2)(1.Fisheries College of Jimei University,Xiamen 361021,China; 2.College of Chemistry & Chemical Engineering,Xiamen University,Xiamen 361005,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12534]
专题	集美大学
推荐引用方式 GB/T 7714	鄢庆枇,邹文政,纪荣兴,等. 应用荧光抗体技术检测牙鲆体内的河流弧菌[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hykx200604003&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	鄢庆枇,邹文政,纪荣兴,庄峙厦,&王小如.(2012).应用荧光抗体技术检测牙鲆体内的河流弧菌.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hykx200604003&dbcode=CJFQ&dbname=CJFQ2006.
MLA	鄢庆枇,et al."应用荧光抗体技术检测牙鲆体内的河流弧菌".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=hykx200604003&dbcode=CJFQ&dbname=CJFQ2006 (2012).