养殖大黄鱼副溶血弧菌的酶联免疫吸附法研究

CORC > 集美大学

	养殖大黄鱼副溶血弧菌的酶联免疫吸附法研究
	王军 ; 鄢庆枇 ; 苏永全 ; 马梁 ; 邵勋
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200103012&dbcode=CJFQ&dbname=CJFQ2001
	2012-06-05 ; 2012-06-05
关键词	酶联免疫吸附法副溶血弧菌大黄鱼 Pseudosciaena crocea Vibrio parahaemolyticus ELISA S943
其他题名	Study on indirect ELISA method for detecting Vibrio parahaemolyticus in cultured Pseudosciaena crocea
中文摘要	本文建立了网箱养殖大黄鱼病原菌———副溶血弧菌酶联免疫吸附法 ,也即间接ELISA快速检测方法 .用 0 .5× 1 0 -3 (V/V)甲醛、30℃作用 8h灭活病原菌制成菌苗 .用该菌苗免疫新西兰兔获得特异性抗血清 ,以辣根过氧化物酶标记羊抗兔IgG为标记第二抗体 ,对副溶血弧菌进行间接ELISA快速检测 ,其最低检测极限为 5×1 0 4CFU/cm3 .现埸检测养殖水体中没有副溶血弧菌检出 ,患病大黄鱼肝脏副溶血弧菌的检出率为 70 % ,外观健康大黄鱼肝脏副溶血弧菌的检出率为 2 0 % .现埸检测结果表明 :间接ELISA检测法不仅可以用于患病大黄鱼病原菌的快速检测 ,而且能够检测出无病症带菌大黄鱼 .; Presented in this paper is a method of indirect Enzyme Linked Immunosorbent Assay (ELISA) for rapid diagnosis of pathogenic V. parahaemolyticus . This pathogen of cultured P. crocea was firstly inactivated to be vaccine by adding formalin 0.5×10 -3 ( V/V ) at 30℃ for 8h, then the antiserum specific for V. parahaemolyticus was produced from rabbits, the anti rabbit IgG goat was marked by horseradish peroxidase (HRP) and used in an indirect ELISA detection. The results showed the lowest V. parahaemolyticus suspension detection limit in laboratory was 5×10 4CFU/cm 3. On worksite, V. parahaemolyticus were detected by ELISA from samples of diseased and healthy P. crocea by 70% and 20% respectively, and none for the seawater. This indicated that ELISA could be used not only to diagnose the clinically diseased P. crocea , but also to recognize pathogen carrier P. crocea .; 【作者单位】厦门大学海洋系、厦门大学亚热带海洋研究所!福建厦门361005；厦门大学海洋系、厦门大学亚热带海洋研究所!福建厦门361005 集美大学水产学院养殖系；福建厦门361021；厦门大学海洋系、厦门大学亚热带海洋研究所!福建厦门；【作者英文名】WANG Jun 1, YAN Qing pi 1,2 ,SU Yong quan 1, MA Liang 1, SHAO Xun 1 (1.Department of Oceanography/ Institute of Subtropical Oceanography, Xiamen University,Xiamen 361005, China; 2.Department of Aquiculture, Fisheries College, Jimei Universit
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12511]
专题	集美大学
推荐引用方式 GB/T 7714	王军,鄢庆枇,苏永全,等. 养殖大黄鱼副溶血弧菌的酶联免疫吸附法研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200103012&dbcode=CJFQ&dbname=CJFQ2001,2012, 2012.
APA	王军,鄢庆枇,苏永全,马梁,&邵勋.(2012).养殖大黄鱼副溶血弧菌的酶联免疫吸附法研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200103012&dbcode=CJFQ&dbname=CJFQ2001.
MLA	王军,et al."养殖大黄鱼副溶血弧菌的酶联免疫吸附法研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=twhx200103012&dbcode=CJFQ&dbname=CJFQ2001 (2012).