高温胁迫下坛紫菜叶状体的生理响应

CORC > 集美大学

	高温胁迫下坛紫菜叶状体的生理响应
	张元 ; 谢潮添 ; 陈昌生 ; 纪德华 ; 周巍巍
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx201103010&dbcode=CJFQ&dbname=CJFQ2011
	2012-06-05 ; 2012-06-05
关键词	坛紫菜高温胁迫生理响应抗逆性 Porphyra haitanensis high temperature stress physiological response stress tolerance S917.3
其他题名	Physiological responses of gametophytic blades of Porphyra haitanensis to rising temperature stresses
中文摘要	以坛紫菜耐高温品系Z-61 F4代叶状体为试验材料、野生型坛紫菜叶状体为对照,研究了高温胁迫(30℃、26℃)与正常培养温度(21℃)下,处理不同天数(0、2、4、6、8、10 d)后坛紫菜叶状体中自由基含量、过氧化氢(H2O2)含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性和游离脯氨酸(Pro)含量的动态变化过程。结果发现耐高温型坛紫菜对高温胁迫的生理响应过程:高温胁迫→活性氧含量上升→细胞膜氧化受损,产生过量膜脂过氧化物→细胞感受到过量的活性氧信号→抗氧化系统和渗透压调节系统共同响应,清除活性氧→自由基含量下降。同时比较两种紫菜对高温胁迫的生理响应过程,发现耐高温型坛紫菜在高温胁迫下能同时启动抗氧化系统和渗透压调节系统,而野生型坛紫菜则只能启动渗透压调节系统,并且两种紫菜抗氧化系统的本底含量也存在着显著差别。由此说明不同品系坛紫菜耐高温性状差异的可能原因是细胞内抗氧化系统的应激性和本底含量存在差异。; Porphyra haitanensis is an economically important marine crop in southern China.Due to global warming it is highly desirable to select or breed new lines of P.haitanensis that can tolerate higher water temperatures;and to use these for cultivation.We observed the physiological responses of gametophytic blades of an F4 high temperature tolerance line(Z-61)and a wild-type line.These were grown at normal temperature(21 ℃),and at high temperatures(26 ℃ and 30 ℃),for 0,2,4,6,8,and 10 days.Changes in physiology were measured,including free radical content,H2O2,malondialdehyde(MDA)and proline levels,and also the activities of superoxide dismutase,peroxidase,and catalase.Physiological changes within the Z-61 gametophytic blades were as follows:high temperature stress→levels of reactive oxygen species(ROS)increased→cell membranes were damaged by oxidation and MDA levels increased→cells received signals of excessive ROS→antioxidation and osmoregulation systems began to clean out excessive ROS→free radical levels declined.We compared the responses of Z-61 and wild-type lines to high temperatures.The Z-61 line is able to initiate both antioxidation and osmoregulation.The wild-type line,however,can only initiate osmoregulation when under high temperature stress.The background activities of the antioxidation systems also show notable differences between the two types.These differences may be the cause that the wild-type is not able to tolerate high temperatures.Our results may lay a foundation for understanding the principles of high temperature tolerance in P.haitanensis,and may suggest some directions for future breeding.; 【作者单位】集美大学水产学院；【作者英文名】ZHANG Yuan,XIE Chao-tian,CHEN Chang-sheng,JI De-hua,ZHOU Wei-wei(College of Fisheries,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12401]
专题	集美大学
推荐引用方式 GB/T 7714	张元,谢潮添,陈昌生,等. 高温胁迫下坛紫菜叶状体的生理响应[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx201103010&dbcode=CJFQ&dbname=CJFQ2011,2012, 2012.
APA	张元,谢潮添,陈昌生,纪德华,&周巍巍.(2012).高温胁迫下坛紫菜叶状体的生理响应.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx201103010&dbcode=CJFQ&dbname=CJFQ2011.
MLA	张元,et al."高温胁迫下坛紫菜叶状体的生理响应".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sckx201103010&dbcode=CJFQ&dbname=CJFQ2011 (2012).