细胞松弛素B诱导鲍异源三倍体

CORC > 集美大学

	细胞松弛素B诱导鲍异源三倍体
	张红云 ; 严正凛 ; 顾勇杰
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201006002&dbcode=CJFQ&dbname=CJFQ2010
	2012-06-05 ; 2012-06-05
关键词	鲍鱼多倍体细胞松弛素B(CB) 异源多倍体 abalone polyploid Cytochalasin B(CB) allotriploid S917.4
其他题名	Allotriploid Abalone Induction by Cytochalasin B
中文摘要	用细胞松弛素B(CB)处理受精卵(九孔鲍♀×盘鲍♂),抑制第二极体释放,诱导异源三倍体.在水温24℃下,分别进行了不同起始处理时间(受精后5~25 min)、不同药物处理浓度(0.2~1.0 mg/L)和不同持续处理时间(5~15 min)的实验.结果表明:受精后10~15 min开始处理的效果较好,平均担轮幼虫成活率为52.07%,平均三倍体率为55.81%;最佳药物处理质量浓度为0.4 mg/L,最佳持续处理时间为10 min,担轮幼虫率为21.43%,异源三倍体率为50.96%;药物空白对照组1(九孔鲍♀×九孔鲍♂)担轮幼虫率为85.38%,二倍体率为95.42%;药物空白对照组2(九孔鲍♀×盘鲍♂)担轮幼虫率为54.37%,二倍体率为75.87%.综合考虑,鲍异源三倍体的适宜诱导条件为:在受精后13 min,即当40%~50%受精卵排出第一极体时,用0.4 mg/L的CB处理10 min.; Fertilized eggs crossing between H.diversicolor aquatilis(♀) and H.discus discus(♂) were treated with Cytochalasin B to induce allotriploid by inhibiting the release of second polarbody.Different treatments of CB,including initial treating time(5～25 mins after fertilization),concentration(0.2～1.0 mg/L) and duration(5～15 min),were tested at 24 ℃ water temperature,and the trochophora survival rate and ploidy rate were calculated.The optimal initial time was 10～15 min after fertilization,which resulted in 52.07 % survival rate of trochophora and 55.81 % allotriploid percentage.The best combination of concentration and duration was 0.4 mg/L CB with 10 min treatment,which resulted in 21.43 % rate of trochophora and 50.96 % allotriploid percentage.The diploid percentage and survival trochophora rate of the group(H.diversicolor aquatilis♀×H.diversicolor aquatilis♂)were 95.42 % and 85.38 % respectively.The diploid percentage and survival trochophora rate of the group(H.diversicolor aquatilis♀×H.discus discus(♂)were 95.42 % and 85.38 %.Overall,the most effective procedure for inducing allotriploid of H.diversicolor aquatilis(♀)×H.discus discus(♂) was to treat fertilized eggs for 10 min by 0.4 mg/L CB the moment(13 mins after fertilization) when 40 %～50 % fertilized eggs released the first polar body.; 【作者单位】集美大学水产学院；福建省高校水产科学技术与食品安全重点实验室；【作者英文名】ZHANG Hong-yun1,YAN Zheng-lin1,2,GU Yong-jie1(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University),Fujian Province,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/11842]
专题	集美大学
推荐引用方式 GB/T 7714	张红云,严正凛,顾勇杰. 细胞松弛素B诱导鲍异源三倍体[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201006002&dbcode=CJFQ&dbname=CJFQ2010,2012, 2012.
APA	张红云,严正凛,&顾勇杰.(2012).细胞松弛素B诱导鲍异源三倍体.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201006002&dbcode=CJFQ&dbname=CJFQ2010.
MLA	张红云,et al."细胞松弛素B诱导鲍异源三倍体".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz201006002&dbcode=CJFQ&dbname=CJFQ2010 (2012).