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	拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应
	王翠丽 ; 田海霞 ; 汪姣姣 ; 齐天从 ; 黄煌 ; 任春梅 ; 谢道昕 ; 宋素胜 ; Cuili Wang ; Haixia Tian ; Jiaojiao Wang ; Tiancong Qi ; Huang Huang ; Chunmei Ren ; Daoxin Xie ; Susheng Song
	2016-03-30 ; 2016-03-30
关键词	b HLH17 COI1 抗性 茉莉素 laser induced breakdown spectroscopy flame atomic absorption by alkali metals Beer-Lambert law thermodynamic equilibrium Q943.2
其他题名	b HLH17 Negatively Regulates Jasmonate-mediated Plant Defense Responses
中文摘要	植物激素茉莉素作为抗性信号调控植物对腐生性病原菌和昆虫的抗性,作为发育信号调控植物根的生长、雄蕊发育、表皮毛形成和叶片衰老。茉莉素受体COI1识别茉莉素分子,进而与JAZ蛋白互作并诱导其降解,继而调控多种茉莉素反应。拟南芥(Arabidopsis thaliana)IIId亚组b HLH转录因子(b HLH3、b HLH13、b HLH14和b HLH17)是JAZ的一类靶蛋白。与野生型相比,IIId亚组b HLH转录因子的单突变体对灰霉菌和甜菜夜蛾的抗性无明显差异,而四突变体对灰霉菌和甜菜夜蛾的抗性增强。该文通过高表达b HLH17并研究其对灰霉菌和甜菜夜蛾的抗性反应,结果显示,被灰霉菌侵染的b HLH17高表达植株较野生型表现出更严重的病症。取食b HLH17高表达植株叶片的甜菜夜蛾幼虫体重大于取食野生型叶片的幼虫体重。b HLH17高表达抑制了茉莉素诱导的抗性相关基因(Thi2.1)和伤害响应基因(VSP2、AOS、JAZ1、JAZ9和JAZ10)的表达。原生质体转化实验显示b HLH17通过其N端行使转录抑制功能。研究结果表明,IIId亚组b HLH转录抑制因子b HLH17高表达会负调控茉莉素介导的对灰霉菌和甜菜夜蛾的抗性。; The plant hormone jasmonate(JA) functions as a defense signal to regulate plant defense against insect attack and pathogen infection. It also acts as a developmental signal to regulate plant development and growth, including root growth, stamen development, trichome initiation, and leaf senescence. The JA receptor CORONATINE INSENSITIVE1(COI1) perceives JA signals for interaction with JASMONATE-ZIM-DOMAIN(JAZ) proteins and subsequent ubiquitination of JAZs, thereby regulating the JA responses. The single mutants of the b HLH subgroup IIId factors(b HLH3, b HLH13, b HLH14 and b HLH17), which interact with JAZs, show no obvious phenotype in resistance to the fungus Botrytis cinerea and defense against the herbivore Spodoptera exigua. However, the quadruple mutant shows enhanced resistance to B. cinerea and defense against S. exigua. We investigated whether the overexpression of b HLH17 in Arabidopsis affected the resistance to B. cinerea and defense against S. exigua. Similar to the JA receptor mutant coi1-1, Arabidopsis b HLH17-overexpressing plants were more susceptible to B. cinerea and S. exigua than the wild type. As compared with the wild type, b HLH17 overexpression attenuated the JA-inducible expression of defense- or wounding-related genes, such as Thi2.1, VSP2, AOS, JAZ1, JAZ9 and JAZ10. Further transient expression analysis revealed that the N-terminus of b HLH17 was essential for its transcriptional repression function. Overexpression of b HLH17 negatively regulates plant defense against B. cinerea and S. exigua.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/148490]
专题	清华大学
推荐引用方式 GB/T 7714	王翠丽,田海霞,汪姣姣,等. 拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应[J],2016, 2016.
APA	王翠丽.,田海霞.,汪姣姣.,齐天从.,黄煌.,...&Susheng Song.(2016).拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应..
MLA	王翠丽,et al."拟南芥转录因子bHLH17负调控茉莉素介导的抗性反应".(2016).

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