QQ客服 官方微博 反馈留言

	Crystal structure of a plant leucine rich repeat protein with two island domains
	SONG Wen ; HAN ZhiFu ; SUN YaDong ; CHAI JiJie ; SONG Wen ; HAN ZhiFu ; SUN YaDong ; CHAI JiJie
	2016-03-30 ; 2016-03-30
关键词	leucine rich repeat receptor-like kinases receptor-like protein kinase 2 Q946
其他题名	Crystal structure of a plant leucine rich repeat protein with two island domains
中文摘要	Leucine rich repeat(LRR)domain,characterized by a repetitive sequence pattern rich in leucine residues,is a universal protein-protein interaction motif present in all life forms.LRR repeats interrupted by sequences of 30 70 residues(termed island domain,ID)have been found in some plant LRR receptor-like kinases(RLKs)and animal Toll-like receptors(TLR7-9).Recent studies provide insight into how a single ID is structurally integrated into an LRR protein.However,structural information on an LRR protein with two IDs is lacking.The receptor-like protein kinase 2(RPK2)is an LRR-RLK and has important roles in controlling plant growth and development by perception and transduction of hormone signal.Here we present the crystal structure of the extracellular LRR domain of RPK2(RPK2-LRR)containing two IDs from Arabidopsis.The structure reveals that both of the IDs are helical and located at the central region of the single RPK2-LRR solenoid.One of them binds to the inner surface of the solenoid,whereas the other one mainly interacts with the lateral side.Unexpectedly,a long loop immediately following the N-terminal capping domain of RPK2-LRR is presented toward and sandwiched between the two IDs,further stabilizing their embedding to the LRR solenoid.A potential ligand binding site formed by the two IDs and the solenoid is located at the C-terminal side of RPK2-LRR.The structural information of RPK2-LRR broadens our understanding toward the large family of LRR proteins and provides insight into RPK2-mediated signaling.; Leucine rich repeat(LRR) domain, characterized by a repetitive sequence pattern rich in leucine residues, is a universal pro- tein-protein interaction motif present in all life forms. LRR repeats interrupted by sequences of 30 70 residues(termed island domain, ID) have been found in some plant LRR receptor-like kinases(RLKs) and animal Toll-like receptors(TLR7-9). Re- cent studies provide insight into how a single ID is structurally integrated into an LRR protein. However, structural infor- mation on an LRR protein with two IDs is lacking. The receptor-like protein kinase 2(RPK2) is an LRR-RLK and has im- portant roles in controlling plant growth and development by perception and transduction of hormone signal. Here we present the crystal structure of the extracellular LRR domain of RPK2(RPK2-LRR) containing two IDs from Arabidopsis. The struc- ture reveals that both of the IDs are helical and located at the central region of the single RPK2-LRR solenoid. One of them binds to the inner surface of the solenoid, whereas the other one mainly interacts with the lateral side. Unexpectedly, a long loop immediately following the N-terminal capping domain of RPK2-LRR is presented toward and sandwiched between the two IDs, further stabilizing their embedding to the LRR solenoid. A potential ligand binding site formed by the two IDs and the solenoid is located at the C-terminal side of RPK2-LRR. The structural information of RPK2-LRR broadens our under- standing toward the large family of LRR proteins and provides insight into RPK2-mediated signaling.
语种	英语 ; 英语
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/148420]
专题	清华大学
推荐引用方式 GB/T 7714	SONG Wen,HAN ZhiFu,SUN YaDong,et al. Crystal structure of a plant leucine rich repeat protein with two island domains[J],2016, 2016.
APA	SONG Wen.,HAN ZhiFu.,SUN YaDong.,CHAI JiJie.,SONG Wen.,...&CHAI JiJie.(2016).Crystal structure of a plant leucine rich repeat protein with two island domains..
MLA	SONG Wen,et al."Crystal structure of a plant leucine rich repeat protein with two island domains".(2016).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。