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	Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli
	Wei, Xiao-Xing ; Shi, Zhen-Yu ; Yuan, Mei-Qing ; Chen, Guo-Qiang
	2010-10-12 ; 2010-10-12
关键词	PHB Polyhydroxyalkanoates Microaerobiosis Anaerobic promoter phbCAB Alcohol dehydrogenase FERMENTATIVE ALCOHOL-DEHYDROGENASE POLY-BETA-HYDROXYBUTYRATE POLY(3-HYDROXYBUTYRATE) SYNTHESIS MOLECULAR-WEIGHT POLYHYDROXYALKANOATE SYNTHASE ETHANOL DEHYDROGENASE DENSITY CULTIVATION RALSTONIA-EUTROPHA ALCALIGENES-LATUS BATCH CULTURE Biotechnology & Applied Microbiology
中文摘要	Nine anaerobic promoters were cloned and constructed upstream of PHB synthesis genes phbCAB from Ralstonia eutropha for the micro- or anaerobic PHB production in recombinant Escherichia coli. Among the promoters, the one for alcohol dehydrogenase (P (adhE) ) was found most effective. Recombinant E. coli JM 109 (pWCY09) harboring P (adhE) and phbCAB achieved a 48% PHB accumulation in the cell dry weight after 48 h of static culture compared with only 30% PHB production under its native promoter. Sixty-seven percent PHB was produced in the dry weight (CDW) of an acetate pathway deleted (Delta pta deletion) E. coli JW2294 harboring the vector pWCY09. In a batch process conducted in a 5.5-l NBS fermentor containing 3 l glucose LB medium, E. coli JW2294 (pWCY09) grew to 7.8 g/l CDW containing 64% PHB after 24 h of microaerobic incubation. In addition, molecular weight of PHB was observed to be much higher under microaerobic culture conditions. The high activity of P (adhE) appeared to be the reason for improved micro- or anaerobic cell growth and PHB production while high molecular weight contributed to the static culture condition.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING ST, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81905]
专题	清华大学
推荐引用方式 GB/T 7714	Wei, Xiao-Xing,Shi, Zhen-Yu,Yuan, Mei-Qing,et al. Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli[J],2010, 2010.
APA	Wei, Xiao-Xing,Shi, Zhen-Yu,Yuan, Mei-Qing,&Chen, Guo-Qiang.(2010).Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli..
MLA	Wei, Xiao-Xing,et al."Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli".(2010).

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