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	Changing the Metal Binding Specificity of Superoxide Dismutase from Thermus thermophilus HB-27 by a Single Mutation
	Wang, Tianwen ; Qiu, Aidong ; Meng, Fanguo ; Zhou, Haimeng
	2010-10-12 ; 2010-10-12
关键词	Superoxide dismutase Active site Molecular modeling Site-directed mutagenesis Folding Tryptophan fluorescence INTERHELICAL HYDROGEN-BONDS PORPHYROMONAS-GINGIVALIS ION SPECIFICITY SEQUENCE SITE CONVERSION PYROGALLOL RESOLUTION DOMAIN ASSAY Biochemistry & Molecular Biology Biotechnology & Applied Microbiology
中文摘要	Metal binding of superoxide dismutase from Thermus thermophilus HB27 was analyzed by comparing the related structures and sequences from different origins. Mutants (Ile166Leu, Asp167Glu, and Ile166Leu-Asp167Glu) were prepared and characterized. The mutants Asp167Glu and Ile166Leu-Asp167Glu changed their binding specificities from manganese to iron, which were manifested by the differences in color of the enzyme solutions and by flame atomic absorption analysis. Specific activities of the three mutants were 112, 52, and 62% of that of the wild-type enzyme, respectively. Asp167Glu and Ile166Leu-Asp167Glu only retained 6.8 and 6.1%, respectively, of the original activities after dialysis against 1 mM EDTA. Tryptophan fluorescence measurement and native gel electrophoresis implied that the three mutants could fold into a less condensed structure. Their folding and changes in the ion binding sites of the modeled structures might be the reason for their low affinities to metal ions. These findings increased our understanding of metal binding specificity of superoxide dismutase.
语种	英语 ; 英语
出版者	HUMANA PRESS INC ; TOTOWA ; 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81900]
专题	清华大学
推荐引用方式 GB/T 7714	Wang, Tianwen,Qiu, Aidong,Meng, Fanguo,et al. Changing the Metal Binding Specificity of Superoxide Dismutase from Thermus thermophilus HB-27 by a Single Mutation[J],2010, 2010.
APA	Wang, Tianwen,Qiu, Aidong,Meng, Fanguo,&Zhou, Haimeng.(2010).Changing the Metal Binding Specificity of Superoxide Dismutase from Thermus thermophilus HB-27 by a Single Mutation..
MLA	Wang, Tianwen,et al."Changing the Metal Binding Specificity of Superoxide Dismutase from Thermus thermophilus HB-27 by a Single Mutation".(2010).

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