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	Action Spectra of Chlorophyll a Biosynthesis in Cyanobacteria: Dark-Operative Protochlorophyllide Oxidoreductase-Deficient Mutants
	Gao, Yang ; Xiong, Wei ; He, Ming J. ; Tang, Li ; Xiang, Jin Y. ; Wu, Qing Y.
	2010-10-12 ; 2010-10-12
关键词	Dark-Operative Pchlide Oxidoreductase (DPOR) Light-Dependent Pchlide Oxidoreductase (LPOR) Synechocystis sp PCC 6803 DNA MICROARRAY ANALYSIS SYNECHOCYSTIS SP PCC-6803 SP STRAIN PCC-6803 RHODOBACTER-CAPSULATUS GENE-EXPRESSION LIGHT PLANTS PHOTOSYNTHESIS IDENTIFICATION RECONSTITUTION Biochemistry & Molecular Biology Pharmacology & Pharmacy
中文摘要	Both light-dependent and light-independent (dark) protochlorophyllide (Pchlide) reductase account for catalyzing the reduction of Pchlide to chlorophyllide during the biosynthesis of Mg-tetrapyrrole pigments in cyanobacteria. To gain more insight into the interaction between the wavelength of the light and these two chlorophyll synthetic pathways in Synechocystis sp. PCC 6803, the spectral effectiveness of the formation of chlorophyll a was investigated during the regreening process in chIL(-) and chlN(-) Mutants, which Could not synthesize chlorophyll during growth in the dark. The action spectra showed obvious maxima around 450 nm and 650 rim, similar to those of higher plants except that the intensities of two peaks are reversed. The mRNA levels of chlL and chlN and chlorophyll a content under different wavelengths of light in the wild-type strain were also measured. The RT-PCR analysis revealed that the transcripts of chlL and chlN were up-regulated in red light but simultaneously down-regulated in green light which resulted in corresponding changes of the chlorophyll content. This fact indicates that the regulation of dark-operative protochlorophyllide oxidoreductase (DPOR) in the transcriptional level is essential for cyanobacteria to synthesize appropriate chlorophyll for acclimating in various light colour environments.
语种	英语 ; 英语
出版者	VERLAG Z NATURFORSCH ; TUBINGEN ; POSTFACH 2645, 72016 TUBINGEN, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81895]
专题	清华大学
推荐引用方式 GB/T 7714	Gao, Yang,Xiong, Wei,He, Ming J.,et al. Action Spectra of Chlorophyll a Biosynthesis in Cyanobacteria: Dark-Operative Protochlorophyllide Oxidoreductase-Deficient Mutants[J],2010, 2010.
APA	Gao, Yang,Xiong, Wei,He, Ming J.,Tang, Li,Xiang, Jin Y.,&Wu, Qing Y..(2010).Action Spectra of Chlorophyll a Biosynthesis in Cyanobacteria: Dark-Operative Protochlorophyllide Oxidoreductase-Deficient Mutants..
MLA	Gao, Yang,et al."Action Spectra of Chlorophyll a Biosynthesis in Cyanobacteria: Dark-Operative Protochlorophyllide Oxidoreductase-Deficient Mutants".(2010).

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