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	Crystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-Pto
	Dong, Jing ; Xiao, Fangming ; Fan, Fenxia ; Gu, Lichuan ; Cang, Huaixing ; Martin, Gregory B. ; Chai, Jijie
	2010-10-12 ; 2010-10-12
关键词	PLANT-DISEASE RESISTANCE AVIRULENCE PROTEIN AVRPTO BACTERIAL SPECK DISEASE E3 UBIQUITIN LIGASE INNATE IMMUNITY CELL-DEATH HYPERSENSITIVE RESPONSE MEDIATED RESISTANCE SYRINGAE PATHOVARS MOLECULAR-BASIS Biochemistry & Molecular Biology Plant Sciences Cell Biology
中文摘要	Resistance to bacterial speck disease in tomato (Solanum lycopersicum) is activated upon recognition by the host Pto kinase of either one of two sequence-unrelated effector proteins, AvrPto or AvrPtoB, from Pseudomonas syringae pv tomato (Pst). Pto induces Pst immunity by acting in concert with the Prf protein. The recently reported structure of the AvrPto-Pto complex revealed that interaction of AvrPto with Pto appears to relieve an inhibitory effect of Pto, allowing Pto to activate Prf. Here, we present the crystal structure of the Pto binding domain of AvrPtoB (residues 121 to 205) at a resolution of 1.9 angstrom and of the AvrPtoB(121-205)-Pto complex at a resolution of 3.3 angstrom. AvrPtoB(121-205) exhibits a tertiary fold that is completely different from that of AvrPto, and its conformation remains largely unchanged upon binding to Pto. In common with AvrPto-Pto, the AvrPtoB-Pto complex relies on two interfaces. One of these interfaces is similar in both complexes, although the primary amino acid sequences from the two effector proteins are very different. Amino acid substitutions in Pto at the other interface disrupt the interaction of AvrPtoB-Pto but not that of AvrPto-Pto. Interestingly, substitutions in Pto affecting this unique interface also cause Pto to induce Prf-dependent host cell death independently of either effector protein.
语种	英语 ; 英语
出版者	AMER SOC PLANT BIOLOGISTS ; ROCKVILLE ; 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81840]
专题	清华大学
推荐引用方式 GB/T 7714	Dong, Jing,Xiao, Fangming,Fan, Fenxia,et al. Crystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-Pto[J],2010, 2010.
APA	Dong, Jing.,Xiao, Fangming.,Fan, Fenxia.,Gu, Lichuan.,Cang, Huaixing.,...&Chai, Jijie.(2010).Crystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-Pto..
MLA	Dong, Jing,et al."Crystal Structure of the Complex between Pseudomonas Effector AvrPtoB and the Tomato Pto Kinase Reveals Both a Shared and a Unique Interface Compared with AvrPto-Pto".(2010).

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