QQ客服 官方微博 反馈留言

	Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum
	Jin, Hui ; Lin, Jin-Ming ; Wang, Xu ; Xin, Tian-bing ; Liang, Shu-xuan ; Li, Zhen-jia ; Hu, Guo-mao
	2010-10-12 ; 2010-10-12
关键词	Free thyroxine Magnetic particles Chemiluminescence enzyme immunoassay Horseradish peroxidase Human serum RADIOIMMUNOASSAY TRIIODOTHYRONINE NANOPARTICLES THYROTROPIN DIALYSIS Chemistry, Analytical Pharmacology & Pharmacy
中文摘要	A magnetic particles-based chemiluminescence enzyme immunoassay with high sensitivity, specificity, rapidity, and reproducibility was developed for the determination of free thyroxine in human serum. A competitive assay has been proposed with horseradish peroxidase labeled thyroxine analog. The immuno-magnetic particles coated with anti-fluorescein isothiocyanate antibody was used as dispersed solid phase and separation means for the immunoassay. Experimental conditions, such as temperature, the volume of magnetic particles and substrate, incubation time, dilution ratio and other relevant variables upon the immunoassay have been examined and optimized. The proposed method exhibited high performance which the linear range was 1.59-122 pmol L-1 and the detection limit was 0.25 pmol L-1. A coefficient of variance of less than 15% was obtained for both intra-assay and inter-assay precision. The present method has been successfully applied to the analysis of free thyroxine in human serum. The diagnostic accordance rate of the method for normal serum, hyperthyroidism, and hypothyroidism are satisfactory. Good correlations were obtained between the results by the proposed method and the commercial radioimmunoassay kit. The present method exhibits good potential in the fabrication of FT4 diagnostic kits which could be used in the clinical analysis and facilitated the development of automated operation systems in the clinical practice. (C) 2009 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/80830]
专题	清华大学
推荐引用方式 GB/T 7714	Jin, Hui,Lin, Jin-Ming,Wang, Xu,et al. Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum[J],2010, 2010.
APA	Jin, Hui.,Lin, Jin-Ming.,Wang, Xu.,Xin, Tian-bing.,Liang, Shu-xuan.,...&Hu, Guo-mao.(2010).Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum..
MLA	Jin, Hui,et al."Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。