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	Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology
	Li, Shengjie ; Xiong, Zhuo ; Wang, Xiaohong ; Yan, Yongnian ; Liu, Haixia ; Zhang, Renji
	2010-10-12 ; 2010-10-12
关键词	rapid prototyping double-nozzle assembling liver regeneration hepatocyte adipose-derived stromal cell tissue engineering vascular-like network hybrid cell/hydrogel construct COLLAGEN/CHITOSAN MATRICES BIOARTIFICIAL LIVERS HEPATIC TISSUE TRANSPLANTATION POLYURETHANE GENERATION HYDROGEL GELATIN SYSTEMS DEVICE Biotechnology & Applied Microbiology Materials Science, Biomaterials Polymer Science
中文摘要	Assembling multitype cells and biomimic extracellular matrix materials in a single construct is a promising method to regenerate complex tissues/organs in vitro. A new double-nozzle assembling technique was used to fabricate a hybrid cell/hydrogel construct with a vascular-like network to perform as a mass exchange conduit. Using a digital model to mimic an anatomical liver structure, adipose-derived stromal cells (ADSC) were combined within a gelatin/alginate/fibrinogen hydrogel to form a vascular-like network and hepatocytes combined gelatin/alginate/chitosan were placed around it. The construct was stabilized in a thrombin/CaCl2/Na5P3O10 solution after assembly. The ADSC were induced to differentiate into endothelial-like cells with endothelial growth factor. The albumin secretion level of the embedded hepatocytes increased during the 2 weeks culture, while the levels of urea and alanine transaminase were decreased after an increasing profile. These preliminary results indicate that this double-nozzle assembling technique could be a powerful tool for fabricating complex constructs with special intrinsic/extrinsic structures. This technology has the potential to be widely used in medical regeneration and drug screening.
语种	英语 ; 英语
出版者	SAGE PUBLICATIONS LTD ; LONDON ; 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/80547]
专题	清华大学
推荐引用方式 GB/T 7714	Li, Shengjie,Xiong, Zhuo,Wang, Xiaohong,et al. Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology[J],2010, 2010.
APA	Li, Shengjie,Xiong, Zhuo,Wang, Xiaohong,Yan, Yongnian,Liu, Haixia,&Zhang, Renji.(2010).Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology..
MLA	Li, Shengjie,et al."Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology".(2010).

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