| A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons | |
| Zhao, B. ; Wang, H. ; Mao, X. ; Li, R. | |
| 2010-10-12 ; 2010-10-12 | |
| 关键词 | cellulose acetate nitrate filter PAH-degrading bacteria polycyclic aromatic hydrocarbons screening method ENUMERATION METABOLISM SITES Biotechnology & Applied Microbiology Microbiology |
| 中文摘要 | Aims: A rapid procedure was developed to screen for bacteria that are able to grow on polycyclic aromatic hydrocarbons (PAHs). Methods and Results: A drop of ethyl ether-dissolved PAH is spread on a sterilized cellulose acetate/nitrate filter lying on the top of a mineral salts agarose plate. After the evaporation of ethyl ether, a serially diluted sample is spread over the filter and incubated. Subsequently, the PAH degrading bacteria can be counted and isolated. Conclusions: This procedure is a simple method for screening bacterial isolates for the ability to grow with PAHs. Significance and Impact of the Study: This technique is rapid to screen and/or count PAH-degrading bacteria and is also used to streak cultivation without disrupting the PAH layer on plate. |
| 语种 | 英语 ; 英语 |
| 出版者 | WILEY-BLACKWELL PUBLISHING, INC ; MALDEN ; COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/79765]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Zhao, B.,Wang, H.,Mao, X.,et al. A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons[J],2010, 2010. |
| APA | Zhao, B.,Wang, H.,Mao, X.,&Li, R..(2010).A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons.. |
| MLA | Zhao, B.,et al."A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons".(2010). |
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