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	Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus
	Yang, Anqi ; Wei, Lei ; Zhao, Weiran ; Xu, Yuanyuan ; Rao, Zihe
	2010-10-12 ; 2010-10-12
关键词	RESPIRATORY SYNDROME CORONAVIRUS REPLICASE PROTEINS Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Crystallography
中文摘要	Avian infectious bronchitis virus (IBV) is a prototype of the group III corona-viruses and encodes 15 nonstructural proteins which make up the transcription/replication machinery. The nsp2 protein from IBV has a unique and novel sequence and has no experimentally confirmed function in replication, whereas it has been proposed to be crucial for early viral infection and may inhibit the early host immune response. The gene that encodes a double-mutant IBV nsp2 N-terminal domain (residues 9-393 of the polyprotein, with mutations Q132L and L270F) was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.5 angstrom resolution and belonged to space group P6(2) or P6(4), with unit-cell parameters a = b = 114.2, c = 61.0 angstrom, alpha = beta = 90, gamma = 120 degrees. Each asymmetric unit contained one molecule.
语种	英语 ; 英语
出版者	WILEY-BLACKWELL PUBLISHING, INC ; MALDEN ; COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78733]
专题	清华大学
推荐引用方式 GB/T 7714	Yang, Anqi,Wei, Lei,Zhao, Weiran,et al. Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus[J],2010, 2010.
APA	Yang, Anqi,Wei, Lei,Zhao, Weiran,Xu, Yuanyuan,&Rao, Zihe.(2010).Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus..
MLA	Yang, Anqi,et al."Expression, crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of nsp2 from avian infectious bronchitis virus".(2010).

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