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	Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model
	Zhang, Han ; Wei, Yue Teng ; Tsang, Kam Sze ; Sun, Chong Ran ; Li, Jin ; Huang, Hua ; Cui, Fu Zhai ; An, Yi Hua
	2010-10-12 ; 2010-10-12
关键词	CULTURED SCHWANN-CELLS EXTRACELLULAR-MATRIX PERIPHERAL-NERVES PROGENITOR CELLS SPINAL-CORD TRANSPLANTATION GRAFTS DIFFERENTIATION ENHANCEMENT ALLOGRAFT Medicine, Research & Experimental
中文摘要	The implantation of neural stem cells (NSCs) in artificial scaffolds for peripheral nerve injuries draws much attention. NSCs were ex-vivo expanded in hyaluronic acid (HA)-collagen composite with neurotrophin-3, and BrdU-labeled NSCs conduit was implanted onto the ends of the transected facial nerve of rabbits. Electromyography demonstrated a progressive decrease of current threshold and increase of voltage amplitude in de-innervated rabbits after implantation for one, four, eight and 12 weeks compared to readouts derived from animals prior to nerve transection. The most remarkable improvement, observed using Electrophysiology, was of de-innervated rabbits implanted with NSCs conduit as opposed to de-innervated counterparts with and without the implantation of HA-collagen, NSCs and HA-collagen, and HA-collagen and neurotrophin-3. Histological examination displayed no nerve fiber in tissue sections of de-innervated rabbits. The arrangement and S-100 immunoreactivity of nerve fibers in the tissue sections of normal rabbits and injured rabbits after implantation of NSCs scaffold for 12 weeks were similar, whereas disorderly arranged minifascicles of various sizes were noted in the other three arms. BrdU(+) cells were detected at 12 weeks post-implantation. Data suggested that NSCs embedded in HA-collagen biomaterial could facilitate re-innervations of damaged facial nerve and the artificial conduit of NSCs might offer a potential treatment modality to peripheral nerve injuries.
语种	英语 ; 英语
出版者	BIOMED CENTRAL LTD ; LONDON ; CURRENT SCIENCE GROUP, MIDDLESEX HOUSE, 34-42 CLEVELAND ST, LONDON W1T 4LB, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78374]
专题	清华大学
推荐引用方式 GB/T 7714	Zhang, Han,Wei, Yue Teng,Tsang, Kam Sze,et al. Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model[J],2010, 2010.
APA	Zhang, Han.,Wei, Yue Teng.,Tsang, Kam Sze.,Sun, Chong Ran.,Li, Jin.,...&An, Yi Hua.(2010).Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model..
MLA	Zhang, Han,et al."Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model".(2010).

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