QQ客服 官方微博 反馈留言

	Viability and Differentiation of Neural Precursors on Hyaluronic Acid Hydrogel Scaffold
	Pan, Linjie ; Ren, Yongjuan ; Cui, Fuzhai ; Xu, Qunyuan
	2010-10-12 ; 2010-10-12
关键词	tissue engineering immunocytochemistry Nogo receptor antibody stem cell culture CNS injury EMBRYONIC STEM-CELLS RAT SPINAL-CORD IN-VITRO NEURITE OUTGROWTH HUMAN NEUROBLASTOMA PROGENITOR-CELL RETINOIC ACID COLLAGEN MATRICES GROWTH Neurosciences
中文摘要	The traditional notion that injured neurons are unable to regenerate in the adult mammalian brain and spinal cord has long been a concern. This view has led to methodology designed to overcome this problem, most recently by advancements in tissue engineering. Here, neural precursor cells (NPCs) and the Nogo receptor antibody (NgR-Ab) or poly-L-lysine (PLL) were tested in concert with hyaluronic acid hydrogel scaffolds (HA). In particular, we wished to optimize viability and differentiation of NPCs within HA hydrogel scaffolds. Our results show that HA hydrogels can be modified physically or chemically to improve NPCs attachment on the scaffolding doped with NgR-Ab or PILL Both the HA hydrogels and their modifications support the viability of NPCs. NPCs were also able to differentiate into neurons and glial cells on HA hydrogels, although this was affected by the different modifications. Immunofluorescence showed that fewer beta-III-tubulin antibody and antineurofilament antibody-positive cells were found on HA-PLL hydrogel compared with HA or HA NgR-Ab hydrogels. This indicates that the PLL-modified HA hydrogels may inhibit differentiation of NPCs, whereas modification by NgR-Ab had no such effect. Finally, the NgR-Ab-modified HA scaffold can be used as not only a NPC delivery system but also a bioactive factor transportation system for CNS repair. (C) 2009 Wiley-Liss, Inc.
语种	英语 ; 英语
出版者	WILEY-LISS ; HOBOKEN ; DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78260]
专题	清华大学
推荐引用方式 GB/T 7714	Pan, Linjie,Ren, Yongjuan,Cui, Fuzhai,et al. Viability and Differentiation of Neural Precursors on Hyaluronic Acid Hydrogel Scaffold[J],2010, 2010.
APA	Pan, Linjie,Ren, Yongjuan,Cui, Fuzhai,&Xu, Qunyuan.(2010).Viability and Differentiation of Neural Precursors on Hyaluronic Acid Hydrogel Scaffold..
MLA	Pan, Linjie,et al."Viability and Differentiation of Neural Precursors on Hyaluronic Acid Hydrogel Scaffold".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。