| 水生植物提取液对噬菌体的化感作用 | |
| 张男男 ; 王震宇 ; 李锋民 ; 胡洪营 ; ZHANG Nan-nan ; WANG Zhen-yu ; LI Feng-min ; HU Hong-ying | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 水生植物 大肠杆菌噬菌体 化感作用 hydrophytes coliphage allelopathy X703 |
| 其他题名 | Allelopathy of Extracts from Hydrophytes on Coliphage |
| 中文摘要 | 为比较不同水生植物的提取液对噬菌体的抑制作用,采用双层琼脂平板计数法研究了水蓼、红蓼、芦苇、芦竹和香蒲等5种水生植物不同部位的提取液对噬菌体T4和f2的化感作用。当提取液浓度为0.5g/L时,水蓼叶对噬菌体T4、f2的抑制率分别达到91%、93%;水蓼茎对T4、f2的抑制率分别为65%、61%;水蓼根对T4、f2的抑制率分别为66%、100%。水蓼根对噬菌体的化感抑制作用最强,对噬菌体T4的半效应浓度(EC50)为0.6mg/L,对噬菌体f2的EC50为7.6mg/L;芦竹茎对噬菌体T4和f2的EC50均为16mg/L,香蒲叶对T4和f2的EC50分别为16mg/L和20mg/L。可见,水蓼具有强抑制噬菌体的能力。; The double agar overlay(DAL)method was used to test the inhibition effect of extracts from hydrophytes on coliphages growth by the plaque forming unit(PFU)assay.The study was made on the allelopathy on two coliphages(T4 and f 2)growth caused by extracts from different parts of five hy-drophytes Polygonum hydropiper,Polygonum orientate,Phragmites communis,Arundo donax and Typha latifolia).When the extract concentration is 0.5 g/L,the inhibition rate(IR)of Polygonum hydropiper leaf on coliphages T4 and f2 are 91 % and 93%,the IR of Polygonum hydropiper stem on coliphages T4 and f2 are 65% and 61%,and the IR of Polysonum hydropiper root on coliphages T4 and f2 are 66% and 100%,respectively.The Polygonum hydropiper root shows the greatest inhibition,whose EC50 for coliphages T4 and f2 are 0.6 mg/L and 7.6 mg/L,respectively.The EC50 of Arundo donax stem for co-liphages T4 and f2 is all 16 mg/L,and the EC50 of Typha latifolia leaf for coliphages T4 and f2 are 16 mg/L and 20 mg/L,respectively.It is concluded that Polygonum hydropiper has strong inhibition effect on the coliphages.; 教育部科学研究重大项目(308016); 国家“十一五”科技支撑计划项目(2006BAC10B03); 国家自然科学基金资助重大国际合作项目(50721140017) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/65101]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 张男男,王震宇,李锋民,等. 水生植物提取液对噬菌体的化感作用[J],2010, 2010. |
| APA | 张男男.,王震宇.,李锋民.,胡洪营.,ZHANG Nan-nan.,...&HU Hong-ying.(2010).水生植物提取液对噬菌体的化感作用.. |
| MLA | 张男男,et al."水生植物提取液对噬菌体的化感作用".(2010). |
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