QQ客服 官方微博 反馈留言

	人工角膜的一体式制备及其增韧方法
	刘妍君 ; 胡平 ; 许波 ; 黄一飞 ; Liu YJ ; Hu P ; Xu B ; Huang YF
	2010-06-10 ; 2010-06-10
关键词	人工角膜 本体聚合 聚(2-羟基)甲基丙烯酸乙酯 一体式 增韧 R318.1
其他题名	Preparation of artificial cornea and its toughening
中文摘要	目的:运用本体聚合的方法一体成型制备人工角膜,解决移植人工角膜手术中多孔裙边和光学中心两部分脱离及被眼球自发排出的问题,以及人工角膜强度的问题。方法:实验于2005-05/2006-05在清华大学化工系高分子研究所和解放军总医院眼科实验室完成。①采取本体聚合的方法来制备人工角膜,一次性制得光学中心和多孔裙边,不使用任何粘结剂。②利用NaCl粒子的大小控制多孔裙边的孔径,采用(2-羟基)甲基丙烯酸乙酯与甲基丙烯酸甲酯本体共聚和制成聚(2-羟基)甲基丙烯酸乙酯-聚氨酯复合物两种方法增强多孔裙边,分别测量了两种方法增强后的人工角膜的撕裂强度。③分别通过白兔角膜移植和大鼠皮下埋植的方法来研究所制备的人工角膜的生物相容性及细胞长入情况。结果:①采用NaCl作为成孔剂,制备的人工角膜多孔裙边中的孔呈现通孔的形态,这种结构非常适合细胞和组织的长入。②与20%(体积百分比)的甲基丙烯酸甲酯共聚改性后材料的撕裂强度是未改性前材料撕裂强度的460%,聚(2-羟基)甲基丙烯酸乙酯-聚氨酯复合物的撕裂强度则可达未改性前材料撕裂强度的1920%。③组织切片表明改性后材料具有良好的生物相容性。结论:在本体聚合制备聚(2-羟基)甲基丙烯酸乙酯人工角膜的基础上,提出两种不同机制的生物相容性多孔裙边增韧方法,大大提高了人工角膜的撕裂强度,可以取代目前溶液聚合制备水凝胶人工角膜的方法。; AIM: Bulk polymerization is used in this paper to prepare the artificial cornea and solve 2 kinds of problems during the artificial cornea replant operation: detachment between porous skirt and central optic region; self-expelled by the eyeball. Additionally, the intensity of artificial cornea is investigated. METHODS: The research was carried out from May 2005 to May 2006 by Institute of Polymer Science and Engineering, Department of Chemistry, Tsinghua University and Department of Ophthalmology, Chinese PLA General Hospital.①Bulk polymerization was used to prepare an artificial cornea with a central optic region surrounded by a porous skirt, which was simultaneously produced without using any adhesives.②The size of pores in the porous skirt was controlled by NaCl particles. Two different ways, bulk copolymerization with methyl methacrylate (MMA) and poly (2-hydroxyethyl methacrylate)-polyurethane (PHEMA-PU) composite, were introduced to toughen the porous skirt, and the tear resistances of two kinds of artificial cornea were tested.③Through the methods of white rabbit cornea replantation and rat subcutaneous burying respectively, we studied the biocompatibility and the cell generation of the prepared artificial cornea. RESULTS: ①NaCl was used as the porosifier to prepare artificial porous skirt and most of the pores in the skirt presented open shapes. This kind of structure was very suitable for the generation of cells and tissues. ②The tear resistance of PHEMA which copolymerized with 20% (volume percentage) MMA was 460% of that of the pure PHEMA. The tear resistance of PHEMA-PU composite was 1 920% of that of the pure PHEMA.③Histological sections revealed that two kinds of toughened porous skirts were quite suitable to be implanted due to good biocompatibility. CONCLUSION: Based on bulk polymerization of PHEMA artificial cornea, we use 2 biocompatibility methods to increase the toughness of porous skirt, which greatly increase the tear resistance of artificial cornea. The methods in this paper can replace the method of commonly used solution polymerization to prepare hydrogel artificial cornea.; 国家自然科学基金资助项目(30471734,30571839)~~
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/63933]
专题	清华大学
推荐引用方式 GB/T 7714	刘妍君,胡平,许波,等. 人工角膜的一体式制备及其增韧方法[J],2010, 2010.
APA	刘妍君.,胡平.,许波.,黄一飞.,Liu YJ.,...&Huang YF.(2010).人工角膜的一体式制备及其增韧方法..
MLA	刘妍君,et al."人工角膜的一体式制备及其增韧方法".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。