| 微流控芯片-激光诱导荧光检测器的研制及核酸片段分离检测中应用 | |
| 刘科辉 ; 梁宁 ; 姚波 ; 罗国安 ; 王义明 ; Liu Kehui ; Liang Ning ; Yao Bo ; Luo Guoan ; Wang Yiming | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 激光诱导荧光 核酸 微流控芯片 噻唑橙单体 Laser-induced fluorescence detector, deoxyribonucleic acid,microfluid chip,To-Pro-3 Q503 |
| 其他题名 | Development of Laser-induced Fluorescence Detector for Deoxyribonucleic Acid Fragments Seperation by Microfluidic Chip |
| 中文摘要 | 采用自行设计的共焦式激光诱导荧光检测器(激发波长635nm,发射波长670nm),以cy5染料对检测器的空间分辨能力和灵敏度进行了测试,检出限达到10-9mol/L。建立了一种微流控芯片快速分离检测DNA片段的方法。以0.8%羟丙基甲基纤维素(HPMC)为筛分介质,以(噻唑橙单体)To-pro-3为荧光标记染料,在玻璃微流控芯片中实现了dsDNA片段的无胶筛分和激光诱导荧光检测,12条DNA片段在75s内得到分离。; A confocal laser-induced fluorescence detector, with λ_ex 635.0 nm and λ_em 670.0 nm, was designed and constructed. The space resolution and sensitivity was examined. The detection limit of Cy5 dye (C_37H_42O_10N_3S_2K) was 10~-9 mol/L. A method was developed for deoxyribonucleic acid (DNA) fragments electrophoresis separation on microfluidic chip. Fragments of dsDNA were separated with non-gel sieving electrophoresis on microfluidic chip, detected by laser-induced fluorescence detector using To-pro-3 dye (a derivative of cyanien dye thiazole orange, structure was shown in the paper).; 国家自然科学基金资助项目(No.202990306) |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/60980]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 刘科辉,梁宁,姚波,等. 微流控芯片-激光诱导荧光检测器的研制及核酸片段分离检测中应用[J],2010, 2010. |
| APA | 刘科辉.,梁宁.,姚波.,罗国安.,王义明.,...&Wang Yiming.(2010).微流控芯片-激光诱导荧光检测器的研制及核酸片段分离检测中应用.. |
| MLA | 刘科辉,et al."微流控芯片-激光诱导荧光检测器的研制及核酸片段分离检测中应用".(2010). |
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