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	应用HPLC-DAD/MS技术评价中药天麻的质量
	张炜 ; 盛彧欣 ; 张金兰 ; 徐锦堂 ; 孙素琴 ; ZHANG Wei ; SHENG Yu-xin ; ZHANG Jin-lan ; XU Jin-tang ; SUN Su-qin
	2010-06-10 ; 2010-06-10
关键词	天麻 指纹图谱 天麻素 天麻苷元 HPLC-DAD/MS Gastrodia elata Bl. fingerprint gastrodin p-hydroxybenzyl alcohol HPLC-DAD/MS R282
其他题名	Evaluation of the quality of Gastrodia elata Bl. by HPLC-DAD/MS
中文摘要	分析比较不同产地及不同炮制方法的天麻药材质量。天麻药材指纹图谱HPLC-DAD分析条件如下:Zorbax XDB C18色谱柱;流动相为乙腈-0.1%醋酸水梯度洗脱,检测波长为270 nm。MS分析条件:采用ESI电离源,负离子检测模式。天麻药材含量测定HPLC条件:流动相为乙腈-0.1%醋酸水等度洗脱,其余均同天麻药材指纹图谱的液相分析条件。结果表明,不同产地天麻药材具有相似的指纹图谱,原药材直接冻干的炮制方法较其他方法具有较高的相似度;指认了天麻药材指纹图谱中8个主要的色谱峰;指纹图谱相似度的分析结果与主要成分定量分析的结果基本一致。本文所建立的HPLC-DAD/MS分析方法能够对天麻药材进行全面的质量评价。; The chromatographic fingerprint of Gastrodia elata Bl.(Tianma) was developed to compare the quality of Tianma samples from different habitats and processing methods.The above analysis method was established by HPLC-DAD technique.And an HPLC method was used to analysis the contents of gastrodin(GAS) and p-hydroxybenzyl alcohol(HBA) in Tianma from different habitats and processed methods.Experiments of chromatographic fingerprint analysis were carried out with a Zorbax XDB C_(18) column(250 mm×4.6 mm,5 μm).The mobile phase consisted of acetonitrile and 0.1% aqueous acetic acid in gradient elution mode.The column was maintained at 25 ℃.Detection was set at 270 nm.The mass spectra were recorded using as ESI source in the negative mode with ion spray voltage at 3 500 V,source temperature at 335 ℃,gas spray at 8.3 kPa and gas flow rate at 9 L·min~(-1).The HPLC methods of quantitative analysis were the same as those of chromatographic fingerprint analysis except the mobile phase,which consisted of acetonitrile and 0.1% aqueous acetic acid in isocratic elution mode with the ratio of 4.5 to 95.5(v/v).Data of chromatographic fingerprint were analyzed by the "similarity evaluation system for chromatographic fingerprint of TCM(Version 2004 A)" software to compare the quality of Tianma.Samples from different habitats with the same processing method were of high similarity,though a few samples showed evident difference in fingerprint graphics.For Tianma samples with different processing methods,the contents of common peaks were different and the processing method of freezing to dry was better than others.With HPLC-MS technique,8 major common peaks in the fingerprint of Tianma were identified by their MS spectra and comparison with the reference standards.The results of similarity analysis for chromatographic fingerprint were basically consistent with those of quantitative analysis. The established HPLC-DAD/MS methods can be used to evaluate the quality of Tianma.; 科技部新技术新方法研究资助项目[科(2004-05)].
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/60943]
专题	清华大学
推荐引用方式 GB/T 7714	张炜,盛彧欣,张金兰,等. 应用HPLC-DAD/MS技术评价中药天麻的质量[J],2010, 2010.
APA	张炜.,盛彧欣.,张金兰.,徐锦堂.,孙素琴.,...&SUN Su-qin.(2010).应用HPLC-DAD/MS技术评价中药天麻的质量..
MLA	张炜,et al."应用HPLC-DAD/MS技术评价中药天麻的质量".(2010).

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