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	聚羟基丁酸己酸酯/聚碳酸亚丙酯共混材料作为血管组织工程支架的性能特征
	张玲 ; 敖强 ; 贺庆 ; 王干 ; 奚静 ; 公衍道 ; 张秀芳 ; Zhang L ; Ao Q ; He Q ; Wang G ; Xi J ; Gong YD ; Zhang XF
	2010-06-10 ; 2010-06-10
关键词	聚羟基丁酸己酸酯 聚碳酸亚丙酯 共混 破裂强度 缝合强度 R318.08
其他题名	Properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/poly propylene carbonate blended materials as a scaffold for blood vessel tissue engineering
中文摘要	目的:为改善生物材料聚羟基丁酸己酸酯(PHBHHx)的力学性能和聚碳酸亚丙酯(PPC)的可加工性,将两者共混,制备不同比例的共混材料,并对共混膜上血管平滑肌细胞的黏附和共混材料制成的管形支架的力学性能进行评价,以选出合适比例的共混材料作为血管组织工程支架。方法:实验于2006-08/2007-04在清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室完成。①制备5种不同比例(PHBHHx∶PPC分别为0∶10,3∶7,5∶5,7∶3,10∶0)的共混材料,利用CCK-8试剂测定兔主动脉平滑肌细胞在共混膜上培养24h后的黏附数量。②采用热致相分离和冷冻干燥法制备管形支架,通过反复浸渍和晾干,在多孔支架外层形成一层晾干的膜层。利用扫描电镜观察支架形貌、压汞仪测量孔隙率和孔径分布,利用自制装置以6.65kPa/s的加压速度测定管形支架的破裂强度,万能材料试验机测定支架的缝合强度,以羊颈动脉为对照。结果:①兔主动脉平滑肌细胞在各种膜上培养24h后,在5∶5比例PHBHHXx/PPC共混膜和PPC膜上相对黏附量高于PHBHHXx膜(P<0.05)。②PHBHHx和共混材料均可制备成管形支架,而单纯PPC支架在制备晾干层时溶化,无法维持原形。管形支架的孔径随PPC含量的增加而增大,孔隙率提高。③支架的破裂强度与羊颈动脉相似(P>0.05);缝合强度均强于羊颈动脉,以5∶5和3∶7PHBHHx/PPC共混材料制备的管形支架的缝合强度高于PHBHHx支架。结论:①与PHBHHx相比,5∶5PHBHHx/PPC共混膜和PPC膜上兔主动脉平滑肌细胞的黏附更好。②共混材料均可以制备成管形支架,并且力学强度符合移植的要求,缝合强度以5∶5和3∶7的PHBHHx/PPC共混材料最佳。; AIM: To improve the mechanical property of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) and processing ability of polypropylene carbonate (PPC), the PHBHHx and PPC were blended in solvent and made into films and tubular scaffolds. The adhesion of rabbit aorta smooth muscle cell (RaSMC) on the films and the mechanical property of tubular scaffolds were evaluated to select a proper material as scaffold for blood vessel tissue engineering. METHODS: The experiment was conducted in the State Key Laboratory of Biomembrane and Membrane Bioengineering, Department of Biological Sciences and Biotechnology, Tsinghua University from August 2006 to April 2007. ①RaSMCs were incubated on the films from different weight ratios of PHBHHx/PPC blended solution (PHBHHx: PPC= 0∶10, 3∶7, 5∶5, 7∶3, and 10∶0) for 24 hours and the amounts of adhesion on different films were detected by CCK-8 kit. ②Tubular scaffolds were prepared using thermally-induced phase separation (TIPS) and freeze-drying methods, and then they were dipped into their solutions respectively and dried in air. A dried membrane was formed on the surface of scaffolds. The morphology of the scaffolds was observed by scanning electron microscopy (SEM); the porosity and pore diameter of the scaffolds were measured with a mercury porosimeter. Mechanical property including burst pressure and suture retention strength was evaluated by self-made instrument at 6.65 kPa/s and omnipotent material testing machine, respectively. Ovine carotid artery served as control. RESULTS: ①After 24 hours, 5∶5 PHBHHx/PPC and PPC films exhibited better RaSMC affinity than PHBHHx alone (P < 0.05). ②After dipping into solutions, PHBHHx and PHBHHx/PPC scaffolds maintained original tubular form with a film layer outside, while porous PPC scaffold dissolved as dipping into PPC solution and drying in air. The pore diameter enlarged and the porosity augmented with the increase of PPC content in the blended solution. ③Burst pressure of the scaffold was similar to that of ovine carotid artery (P > 0.05), and suture retention strength was superior to that of ovine carotid artery. Moreover, the suture retention strength of PHBHHx/PPC scaffolds at ratio of 5∶5 and 3∶7 was higher than that of PHBHHx scaffold. CONCLUSION: ①Adhesion of RaSMC on 5∶5 PHBHHx/PPC and PPC films is better than PHBHHx film. ②PHBHHx and PHBHHx/PPC blended solution can be prepared into porous tubular scaffold with a film layer outside. The mechanical properties of the scaffolds are appropriate for implantation, especially that of 5∶5 and 3∶7 PHBHHx/PPC scaffolds.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/60582]
专题	清华大学
推荐引用方式 GB/T 7714	张玲,敖强,贺庆,等. 聚羟基丁酸己酸酯/聚碳酸亚丙酯共混材料作为血管组织工程支架的性能特征[J],2010, 2010.
APA	张玲.,敖强.,贺庆.,王干.,奚静.,...&Zhang XF.(2010).聚羟基丁酸己酸酯/聚碳酸亚丙酯共混材料作为血管组织工程支架的性能特征..
MLA	张玲,et al."聚羟基丁酸己酸酯/聚碳酸亚丙酯共混材料作为血管组织工程支架的性能特征".(2010).

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