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	川芎嗪对脑缺血下大鼠纹状体区痕量氨基酸类神经递质的影响
	党红梅 ; 马万云 ; 韩慧婉 ; DANG Hong-Mei ; MA Wan-Yun ; HAN Hui-Wan
	2010-06-07 ; 2010-06-07
关键词	激光诱导荧光 微透析 氨基酸类神经递质 脑缺血 纹状体 Laser-induced fluorescence Microdialysis Amino acid neurotramsmitter Cerebral ischemia Striatum R285
其他题名	Influence of Ligustrazine on Trace Amino Acid Neurotransmitters in Striatum of Rats after Transient Focal Cerebral Ischemia by CZE-LIF
中文摘要	采用脑内微透析技术结合激光诱导荧光检测的高效毛细管电泳研究了脑缺血下盐酸川芎嗪注射液对大鼠纹状体区氨基酸类神经递质含量的影响.在优化的分离条件下,各氨基酸在1×10-6~1×10-8mol/L的浓度范围内与荧光强度呈良好的线性关系,线性相关系数达到0.99以上,检测限低于1.6×10-9mol/L,适合于大鼠纹状体区微透析样品中的痕量氨基酸类神经递质的测量.实验结果表明,脑缺血时,使用盐酸川芎嗪注射液可显著降低细胞外兴奋性氨基酸神经递质谷氮酸(G lu)和天门冬氨酸(Asp)的浓度(P<0.05),同时可显著升高抑制性氨基酸神经递质γ-氨基丁酸(GABA)和甘氨酸(G ly)的浓度(P<0.05).这一结果将对阐明盐酸川芎嗪注射液治疗缺血性脑血管疾病的有关机理提供帮助.; An effective coupling of microdialysis and capillary electrophoresis with laser induced fluorescence detection technique(CZE-LIF) was explored for the analysis of amino acid neurotransmitters in striatum of rat after transient focal cerebral ischemia in group treated with saline and group treated with hydrochloric acid(ligustrazine).The separation of amino acids was achieved within 30 min on an uncoated fused silica capillary((67 cm×)50 μm i.d.) column with botate buffer(10 mmol/L,pH=10.35) and an applied voltage of(12.5 kV).With the current method,the fluorescence intensity has linear relationship with each amino acid concentration between 1×10~-6—1×10~-8 mol/L and the correlation coefficient is above 0.99.The detection limit is superior to(1.6×)10~-9 mol/L for each amino acid.It was sufficiently sensitive and rapid for determination of amino acid in microdialysate.The determination results show that in case of ischemia,concentration of Glu and Asp in group treated with hydrochloric acid ligustrazine were lower than those in the group treated with saline(P<0.05), and hydrochloric acid ligustrazine injection could increase obviously the concentration of GABA and Gly(P<0.05).The results contribute to clarifying the mechanism of hydrochloric acid ligustrazine injection in curing ischemic cerebrovascular diseased,and suggest that CZE-LIF with microdialysis was a powerful method applied to quantitative analysis of trace amino acid neurotransmitters in brain.; 国家自然科学基金(批准号:10274042,10474054)资助.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/40904]
专题	清华大学
推荐引用方式 GB/T 7714	党红梅,马万云,韩慧婉,等. 川芎嗪对脑缺血下大鼠纹状体区痕量氨基酸类神经递质的影响[J],2010, 2010.
APA	党红梅,马万云,韩慧婉,DANG Hong-Mei,MA Wan-Yun,&HAN Hui-Wan.(2010).川芎嗪对脑缺血下大鼠纹状体区痕量氨基酸类神经递质的影响..
MLA	党红梅,et al."川芎嗪对脑缺血下大鼠纹状体区痕量氨基酸类神经递质的影响".(2010).

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