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	磁性微粒子化学发光免疫分析法测定人血清中雌三醇
	吴丹凝 ; 王栩 ; 林金明 ; 李振甲 ; 应希堂 ; WU Dan-Ning ; WANG Xu ; LIN Jin-Ming ; LI Zhen-Jia ; YING Xi-Tang
	2010-06-07 ; 2010-06-07
关键词	雌三醇 磁性微粒子 化学发光免疫分析法 人血清 estriol magnetic microparticle chemiluminescence immunoassay human serum R446.6
其他题名	Determination of Estriol in Human Serum by a Chemiluminescence Immunoassay Method Using Magnetic Microparticle
中文摘要	利用化学发光免疫分析(chemiluminescence immunoassay,CLIA)高灵敏度和高特异性的特点,将磁性微粒子应用于化学发光免疫分析中,用两种不同的方法对人体血清内的雌三醇(E3)含量进行了测定.磁性微粒子分别作为固相一抗包被材料和二抗分离剂参与反应.两种方法检测雌三醇浓度的线形范围均为0.6～60ng/mL.其中,固相一抗法的批内变异及批间变异系数分别小于11%和15%,回收率为90%～116%,健全性系数为0.9987.二抗分离法的批内变异及批间变异系数分别小于8%和10%,回收率为88%～118%,健全性系数为0.9974.两种方法分别与经典板式化学发光法对比,检测人血清样本,结果相关性较好,且磁性微粒子法更为省时、简便,适于推广应用.; A chemiluminescence immunoassay (CLIA) method of high sensitivity, selectivity and reproducibility for the determination of estriol (E3) in human serum was developed. The magnetic microparticles were used as the second-antibody separation agent and the first-antibody solid-surface, respectively. Under the selected conditions, the two methods with magnetic microparticles as the second-antibody separation agent and the first-antibody solid-surface respectively, showed a calibration curve for E3 both in the range of 0.6～60 ng/mL. In the first-antibody method, the CVs (coefficient of variations) of intra- and inter-assays were less than 11% and 15%, respectively. The recovery was in the range of 90%～116%, and the soundness coefficient was 0.9987. In the second-antibody method, the CVs of intra- and inter-assays were less than 8% and 10%, respectively. The recovery was in the range of 88%～118%, and the soundness coefficient was 0.9974. Compared with the traditional micro-plate CLIA method, both of the proposed methods exhibited less analysis time and labor. The proposed methods have been used to determine E3 in human serum successfully.; 国家自然科学基金(No.20437020); 教育部创新团队(No.IRT0404)资助项目.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/40542]
专题	清华大学
推荐引用方式 GB/T 7714	吴丹凝,王栩,林金明,等. 磁性微粒子化学发光免疫分析法测定人血清中雌三醇[J],2010, 2010.
APA	吴丹凝.,王栩.,林金明.,李振甲.,应希堂.,...&YING Xi-Tang.(2010).磁性微粒子化学发光免疫分析法测定人血清中雌三醇..
MLA	吴丹凝,et al."磁性微粒子化学发光免疫分析法测定人血清中雌三醇".(2010).

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