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	Spectroscopic studies of interactions involving horseradish peroxidase and Tb3+
	Guo, Shaofen ; Zhou, Qing ; Lu, Tianhong ; Ding, Xiaolan ; Huang, Xiaohua
	2010-05-11 ; 2010-05-11
关键词	Tb3+ horseradish peroxidase conformation microstructure catalytic activity RARE-EARTH ELEMENTS RAY PHOTOELECTRON-SPECTROSCOPY CONFORMATIONAL-CHANGES CIRCULAR-DICHROISM ELECTROCHEMICAL REACTION TRYPTOPHAN FLUORESCENCE PHYSIOLOGICAL SOLUTION LANTHANIDE IONS CYTOCHROME-C PH Spectroscopy
中文摘要	The spectroscopic properties of interactions involving horseradish peroxidase (HRP) and W, in the simulated physiological solution was investigated with some electrochemical and spectroscopic methods, such as cyclic voltammetry (CV), circular dichroism (CD), X-ray photoelectron spectroscopy (XPS) and synchronous fluorescence (SF). It was found that Tb3+ can coordinate with oxygen atoms in carbonyl groups in the peptide chain of HRP, form the complex of Tb3+ and HRP (Tb-HRP). and then lead to the conformation change of HRP. The increase in the random coil content of HRP can disturb the microstructure of the heme active center of HRP, in which the planarity of the porphyrin cycle in the heme group is increased and then the exposure extent of the electrochemical active center is decreased. Thus W, can inhibit the electrochemical reaction of HRP and its electrocatalytic activity for the reduction of H2O2 at the Au/Cys/GC electrode. The changes in the microstructure of HRP obstructed the electron transfer of Fe(III) in the porphyrin cycle of the heme group, thus HRP catalytic activity is inhibited. The inhibition effect of Tb3+ on HRP catalytic activity is increased with the increasing of Tb3+ concentration. This study would provide some references for better understanding the rare earth elements and heavy metals on peroxidase toxicity in living organisms. (D 2007 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26927]
专题	清华大学
推荐引用方式 GB/T 7714	Guo, Shaofen,Zhou, Qing,Lu, Tianhong,et al. Spectroscopic studies of interactions involving horseradish peroxidase and Tb3+[J],2010, 2010.
APA	Guo, Shaofen,Zhou, Qing,Lu, Tianhong,Ding, Xiaolan,&Huang, Xiaohua.(2010).Spectroscopic studies of interactions involving horseradish peroxidase and Tb3+..
MLA	Guo, Shaofen,et al."Spectroscopic studies of interactions involving horseradish peroxidase and Tb3+".(2010).

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