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	OsDREB1 gene from rice enhances cold tolerance in tobacco
	Li Ping ; Chen Feng ; Quan Chao ; Zhang Guiyou
	2010-05-11 ; 2010-05-11
关键词	Practical/ biotechnology biothermics botany crops genetic engineering proteins/ OsDREB1 gene expression rice cold tolerance tobacco plants DREBP transcription factor subfamily cold stresses dehydration-responsive element binding proteins CBF1 DREB1A DREB2A Arabidopsis thaliana cloning Agrobacterium-mediated transfer method transgenic plants polymerase chain reaction PCR analysis tobacco genome freezing stress experiments/ E3628 Biotechnology industry E3010 Agriculture
中文摘要	The OsDREB1 gene from rice encodes a transcription factor belonging to the DREBP transcription factor subfamily. Many DREBP transcription factors regulate gene expression in response to drought, high-salt, and cold stresses by binding specifically to the dehydration-responsive element (DRE). DRE-binding proteins, such as CBF1, DREB1A, and DREB2A, have been cloned from Arabidopsis thaliana and have been proved to play an important role in stress response of Arabidopsis and several other plants. In this study, the OsDREB1 gene was transferred to tobacco plants by the Agrobacterium-mediated transfer method, and 16 transgenic plants were identified. PCR analysis demonstrates that the foreign genes have been integrated into the tobacco genome. Results of freezing stress experiments indicate that the transgenic plants have enhanced cold tolerance.
语种	英语 ; 英语
出版者	Editorial Board of J. of Tsinghua University ; China
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26906]
专题	清华大学
推荐引用方式 GB/T 7714	Li Ping,Chen Feng,Quan Chao,et al. OsDREB1 gene from rice enhances cold tolerance in tobacco[J],2010, 2010.
APA	Li Ping,Chen Feng,Quan Chao,&Zhang Guiyou.(2010).OsDREB1 gene from rice enhances cold tolerance in tobacco..
MLA	Li Ping,et al."OsDREB1 gene from rice enhances cold tolerance in tobacco".(2010).

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