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	Microbial production of L-glutamate and L-glutamine by recombinant Corynebacterium glutamicum harboring Vitreoscilla hemoglobin gene vgb
	Liu, Qian ; Zhang, Jiao ; Wei, Xiao-Xing ; Ouyang, Shao-Ping ; Wu, Qiong ; Chen, Guo-Qiang
	2010-05-11 ; 2010-05-11
关键词	Corynebacterium glutamicum L-glutamate L-glutamine Vitreoscilla hemoglobin vgb ESCHERICHIA-COLI INTRACELLULAR EXPRESSION INDUSTRIAL-PRODUCTION AEROMONAS-HYDROPHILA NAD(P)H FLUORESCENCE PROMOTER METABOLISM PROTEINS IMPROVES CLONING Biotechnology & Applied Microbiology
中文摘要	Vitreoscilla hemoglobin (VHb) gene vgb equipped with a native promoter Pvgb or a tac promoter Ptac was introduced into Corynebacterium glutamicum ATCC14067, respectively. Ptac was proven to be more suitable for expressing VHb protein in higher concentration in both Escherichia coli and C. glutamicum strains compared with the native vgb promoter Pvgb. VHb-expressing C. glutamicum exhibited higher oxygen uptake rate and enhanced cell growth. Recombinant C. glutamicum harboring vgb gene equipped with Ptac promoter produced 23% more l-glutamate in shake-flask culture and grew to 30% more cell density and formed 22% more l-glutamate in fermentor studies compared with the wild-type strain. When a site-directed mutagenesis in which Tyr405 was replaced by a phenylalanine residue (Y405F) was performed on glutamine synthesis gene, recombinant C. glutamicum overexpressing the mutated gene glnA' was able to produce L-glutamine effectively. Co-expression of vgb and glnA' genes in C. glutamicum produced 17 g/l L-glutamine in shake flask culture, approximately 30% more than that produced by the recombinant harboring only glnA' gene. In fermentor cultivation, the recombinant yielded 25% more cells and produced 40.5 g/l L-glutamine. In this study, it was clearly demonstrated that VHb significantly enhanced cell growth, L-glutamate, and L-glutamine production by recombinant C. glutamicum.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING STREET, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26904]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, Qian,Zhang, Jiao,Wei, Xiao-Xing,et al. Microbial production of L-glutamate and L-glutamine by recombinant Corynebacterium glutamicum harboring Vitreoscilla hemoglobin gene vgb[J],2010, 2010.
APA	Liu, Qian,Zhang, Jiao,Wei, Xiao-Xing,Ouyang, Shao-Ping,Wu, Qiong,&Chen, Guo-Qiang.(2010).Microbial production of L-glutamate and L-glutamine by recombinant Corynebacterium glutamicum harboring Vitreoscilla hemoglobin gene vgb..
MLA	Liu, Qian,et al."Microbial production of L-glutamate and L-glutamine by recombinant Corynebacterium glutamicum harboring Vitreoscilla hemoglobin gene vgb".(2010).

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