| Zinc up-regulated the expression of the rice metallonthionein gene family and enhanced the zinc tolerance of yeast cells | |
| Xu Yufeng ; Zhou Gongke ; Li Yiqin ; Liu Jinyuan | |
| 2010-05-11 ; 2010-05-11 ; OCT | |
| 关键词 | rice metallothionein induced expression Zn2+ tolerance METALLOTHIONEIN GENE DIFFERENTIAL EXPRESSION ARABIDOPSIS-THALIANA PROTEIN HOMEOSTASIS METABOLISM RESISTANCE PATTERNS CLONING ROLES Multidisciplinary Sciences |
| 中文摘要 | Northern blot and functional complementation assay were employed to analyze the effects of zinc on expression of ten rice metallothionein genes (OsMT-Is) in rice seedlings and the growth of yeast cells transformed with OsMT-Is. Northern blot revealed that in shoots of the rice seedlings treated with different Zn2+ concentrations, expression of most members of OsMT-I family was increased, except the type 4 OsMT-Is (OsMT-I-4a, 4b and 4c). In roots, Zn2+ significantly increased the transcription of OsMT-I-1b and OsMT-I-2c, but reduced the trascription of OsMT-I-1a and OsMT-I-3a. When these ten cDNAs were heterologously expressed in zinc sensitive yeast mutant, all transgenic yeasts showed increased tolerance to Zn2+, and zinc accumulation in these yeast cells also increased. These indicated that OsMT-I family members might respond to extra Zn2+, and they could enhance Zn2+ tolerance of cells by direct binding Zn2+. |
| 语种 | 英语 ; 英语 |
| 出版者 | ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/26625]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Xu Yufeng,Zhou Gongke,Li Yiqin,et al. Zinc up-regulated the expression of the rice metallonthionein gene family and enhanced the zinc tolerance of yeast cells[J],2010, 2010, OCT. |
| APA | Xu Yufeng,Zhou Gongke,Li Yiqin,&Liu Jinyuan.(2010).Zinc up-regulated the expression of the rice metallonthionein gene family and enhanced the zinc tolerance of yeast cells.. |
| MLA | Xu Yufeng,et al."Zinc up-regulated the expression of the rice metallonthionein gene family and enhanced the zinc tolerance of yeast cells".(2010). |
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