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	Identification of Festuca arundinacea Schreb Cat1 catalase gene and analysis of its expression under ablotic stresses
	Yang, WL ; Liu, JM ; Chen, F ; Liu, Q ; Gong, YD ; Zhao, NM
	2010-05-11 ; 2010-05-11
关键词	catalase abiotic stress Festuca arundinacea reactive oxygen species NICOTIANA-PLUMBAGINIFOLIA L PEROXISOMAL TARGETING SIGNAL ABSCISIC-ACID ARABIDOPSIS-THALIANA SALT STRESS HIGH LIGHT MAIZE RESPONSES CLONING RESISTANCE Biochemistry & Molecular Biology Plant Sciences
中文摘要	Abiotic stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very important role in plants. To characterize the catalase involved in plant response to abiotic stresses, we constructed a cDNA library from 4 degrees C-treated Festuca arundinacea Schreb seedlings and isolated a catalase gene from this library. The cDNA (FaCat1, 1 735 bp) contained an open reading frame of 1 479 bp. BLAST analysis indicated that the deduced amino acid sequence showed 96% identity with that from wheat TaCat1 and 87% identity with that from maize ZmCat2. Northern blotting analysis showed an obvious increase of FaCat1 transcripts in leaves in contrast with roots. Time-course analysis of the expression of FaCat1 in F. arundinacea leaves showed that FaCat1 expression was upregulated in cold- and salt-stressed leaves, with the FaCat1 transcripts accumulating mostly at 4 or 2 h after cold or salt stress, respectively. No significant changes in FaCat1 transcription were observed in dried leaves and inhibition of FaCat1 transcription was found in abscisic acid (ABA)-treated leaves, indicating that the FaCat1 gene is differentially expressed during cold, high salt, drought, and ABA treatment in F. arundinacea leaves.
语种	英语 ; 英语
出版者	BLACKWELL PUBLISHING ; OXFORD ; 9600 GARSINGTON RD, OXFORD OX4 2DQ, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26612]
专题	清华大学
推荐引用方式 GB/T 7714	Yang, WL,Liu, JM,Chen, F,et al. Identification of Festuca arundinacea Schreb Cat1 catalase gene and analysis of its expression under ablotic stresses[J],2010, 2010.
APA	Yang, WL,Liu, JM,Chen, F,Liu, Q,Gong, YD,&Zhao, NM.(2010).Identification of Festuca arundinacea Schreb Cat1 catalase gene and analysis of its expression under ablotic stresses..
MLA	Yang, WL,et al."Identification of Festuca arundinacea Schreb Cat1 catalase gene and analysis of its expression under ablotic stresses".(2010).

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