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	Collagen nanofiber-covered porous biodegradable carboxymethyl chitosan microcarriers for tissue engineering cartilage
	Lu, Guangyuan ; Sheng, Baiyang ; Wei, Yujun ; Wang, Gan ; Zhang, Lihai ; Ao, Qiang ; Gong, Yandao ; Zhang, Xiufang
	2010-05-11 ; 2010-05-11
关键词	Polymeric microcarriers Carboxymethyl chitosan Collagen nanofibers Cartilage tissue engineering BOVINE CHONDROCYTES 3-DIMENSIONAL CULTURE IN-VITRO SCAFFOLDS BONE DEGRADATION MICROSPHERES BIOMATERIALS REGENERATION EXPRESSION Polymer Science
中文摘要	Nanofibrous collagen-coated porous carboxymethyl chitosan microcarriers (CMC-MCs) were successfully fabricated for use as injectable cell microcarriers. A modified phase separation method combined with temperature controlled freeze-extraction was used for formulating the CMC-MCs. Collagen nanofibers were immobilized onto the surfaces of the CMC-MCs via covalently anchoring some collagen molecules first and more molecules self-assembling into nano-scale fibrous networks afterward. Scanning electron microscopy and hydroxyproline colorimetry analysis revealed that more collagen was immobilized on the CMC-MCs with collagen molecules anchored initially. In vitro cell culture revealed that chondrocytes could adhere, proliferate, and remain differentiated on the nanofiber-coated CMC-MCs. Optical microscopy and confocal laser scanning microscopy showed that chondrocytes grew to confluence on the CMC-MCs within 3 days post-seeding. Subsequently, several confluent CMC-MCs attached to each other, forming tissue-like aggregates after 7 days culture. The mRNA expression of type II collagen was much stronger in chondrocytes cultured on the nanofiber-coated CMC-MCs for 7 days than those cultured in 24-well plates or on CMC-MCs without initial treatment. These porous CMC-MCs could be utilized for cultivating cells and for application in cartilage tissue engineering as injectable scaffolds for cell delivery. (C) 2008 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26606]
专题	清华大学
推荐引用方式 GB/T 7714	Lu, Guangyuan,Sheng, Baiyang,Wei, Yujun,et al. Collagen nanofiber-covered porous biodegradable carboxymethyl chitosan microcarriers for tissue engineering cartilage[J],2010, 2010.
APA	Lu, Guangyuan.,Sheng, Baiyang.,Wei, Yujun.,Wang, Gan.,Zhang, Lihai.,...&Zhang, Xiufang.(2010).Collagen nanofiber-covered porous biodegradable carboxymethyl chitosan microcarriers for tissue engineering cartilage..
MLA	Lu, Guangyuan,et al."Collagen nanofiber-covered porous biodegradable carboxymethyl chitosan microcarriers for tissue engineering cartilage".(2010).

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