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	Microbial production of R-3-hydroxybutyric acid by recombinant E-coli harboring genes of phbA, phbB, and tesB
	Liu, Qian ; Ouyang, Shao-Ping ; Chung, Ahleum ; Wu, Qiong ; Chen, Guo-Qiang
	2010-05-11 ; 2010-05-11
关键词	PHB 3-hydroxybutyric acid 3HB thioesterase II tesB beta-ketothiolase phbA acetoacetyl CoA reductase phbB 3-HYDROXYDECANOIC ACID ENHANCED PRODUCTION THIOESTERASE-II BIOSYNTHESIS POLY(3-HYDROXYALKANOATES) POLYHYDROXYALKANOATES PROLIFERATION PURIFICATION DEGRADATION CLONING Biotechnology & Applied Microbiology
中文摘要	Production of R-3-hydroxybutyric acid (3HB) was observed when genes of beta-ketothiolase (PhbA), acetoacetyl CoA reductase (PhbB), and thioesterase II (TesB) were jointly expressed in Escherichia coli. TesB, generally regarded as a medium chain length acyl CoA thioesterase, was found, for the first time, to play an important role for transforming short chain length 3-hydroxybutyrate-CoA to its free fatty acid, namely, 3HB. E. coli BW25113 (pSPB01) harboring phbA, phbB, and tesB genes produced approximately 4 g/l 3HB in shake flask culture within 24 h with glucose used as a carbon source. Under anaerobic growth conditions, 3HB production was found to be more effective, achieving 0.47 g 3HB/g glucose compared with only 0.32 g 3HB/g glucose obtained from aerobic process. When growth was conducted on sodium gluconate, 6 g/l 3HB was obtained. In a 24-h fed-batch growth process conducted in a 6-l fermentor containing 3 l glucose mineral medium, 12 g/l 3HB was produced from 17 g/l cell dry weight (CDW). This was the highest 3HB productivity achieved by a one-stage fermentation process for 3HB production.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING STREET, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26526]
专题	清华大学
推荐引用方式 GB/T 7714	Liu, Qian,Ouyang, Shao-Ping,Chung, Ahleum,et al. Microbial production of R-3-hydroxybutyric acid by recombinant E-coli harboring genes of phbA, phbB, and tesB[J],2010, 2010.
APA	Liu, Qian,Ouyang, Shao-Ping,Chung, Ahleum,Wu, Qiong,&Chen, Guo-Qiang.(2010).Microbial production of R-3-hydroxybutyric acid by recombinant E-coli harboring genes of phbA, phbB, and tesB..
MLA	Liu, Qian,et al."Microbial production of R-3-hydroxybutyric acid by recombinant E-coli harboring genes of phbA, phbB, and tesB".(2010).

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