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	Layer-by-layer assembly of polyelectrolyte films improving cytocompatibility to neural cells
	Wu, Zhong-Rong ; Ma, Jun ; Liu, Bing-Fang ; Xu, Qun-Yuan ; Cui, Fu-Zhai
	2010-05-10 ; 2010-05-10
关键词	polyelectrolyte multi-layers hyaluronic acid surface roughness cell adhesion neurite outgrowth RAT HIPPOCAMPAL-NEURONS SERUM-FREE CULTURE MULTILAYER FILMS HYALURONIC-ACID ENDOTHELIAL-CELL FORCE MICROSCOPY ADHESION COLLAGEN SURFACE BRAIN Engineering, Biomedical Materials Science, Biomaterials
中文摘要	The using of layer-by-layer assembly polyelectrolyte (PE) films has been suggested as a new versatile technique for surface modification aimed at tissue engineering and cell-based chips. In this study, we investigated the surface morphology of the hyaluronic acid (HA)-based PE films deposited on the amino-functionalized glass slides using atomic force microscopy. These thin films (bilayer number < 9) were measured to have nanoscale roughness ranging from 10 to 100 nm. Then the primary hippocampal and cortical neural cells were cultured on the PE films, respectively. After 5 days of culturing, the cytocompatibility to neural cells was evaluated by cellular morphology, neurite outgrowth, and microtubule-associated protein 2 expressions. From the present results, the HA-based PE films were found to be able to support neural cell adhesion and neurite development, especially for the polycation-ending films. It is suggested these HA-based multilayer PE films or similar build-ups could thus be used in the future as a way to modify surfaces for nerve scaffolds and neuron-based chips. (c) 2006 Wiley Periodicals, Inc.
语种	英语 ; 英语
出版者	WILEY-LISS ; HOBOKEN ; DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/20732]
专题	清华大学
推荐引用方式 GB/T 7714	Wu, Zhong-Rong,Ma, Jun,Liu, Bing-Fang,et al. Layer-by-layer assembly of polyelectrolyte films improving cytocompatibility to neural cells[J],2010, 2010.
APA	Wu, Zhong-Rong,Ma, Jun,Liu, Bing-Fang,Xu, Qun-Yuan,&Cui, Fu-Zhai.(2010).Layer-by-layer assembly of polyelectrolyte films improving cytocompatibility to neural cells..
MLA	Wu, Zhong-Rong,et al."Layer-by-layer assembly of polyelectrolyte films improving cytocompatibility to neural cells".(2010).

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