QQ客服 官方微博 反馈留言

	Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) production in recombinant Aeromonas hydrophila 4AK4 harboring phbA, phbB and vgb genes
	Ouyang, SP ; Han, J ; Qiu, YZ ; Qin, LF ; Chen, S ; Wu, Q ; Leski, ML ; Chen, GQ
	2010-05-10 ; 2010-05-10
会议名称	MACROMOLECULAR SYMPOSIA ; 8th World Conference on Biodegradable Polymers and Plastics ; Seoul, SOUTH KOREA ; Web of Science ; INSPEC
关键词	Aeromonas hydrophila hemoglobin PHB polyhydroxyalkanoates vgb VITREOSCILLA HEMOGLOBIN GENE ESCHERICHIA-COLI CELL-GROWTH BIOSYNTHESIS POLY(BETA-HYDROXYBUTYRATE) EXPRESSION SCAFFOLDS PATHWAY CLONING CAVIAE Polymer Science
中文摘要	Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), a copolyester consisting of 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HHx), can be synthesized by Aeromonas hydrophila strain 4AK4 using long chain fatty acids as the carbon source. The wild type A. hydrophila 4AK4 accumulated PHBHHx consisting of 12-15 mol% 3HHx regardless of growth conditions. When phbA, phbB and vgb genes encoding beta-ketothiolase, acetoacetyl-CoA reductase and vitreoscilla hemoglobin, respectively, were introduced together into A. hydrophila 4AK4, the recombinant strain grew to over 20 g/L cell dry weight (CDW) after 48 h of shake flask cultivation in cosubstrates of dodecanoate and gluconate (weight ratio 1: 1), and the CDW contained 50% PHBHHx consisting of 9 mol% 3HHx. Under similar conditions, the wild type strain produced only 12 g/L CDW containing 32% PHBHHx with 15 mol% 3HHx. In comparison, recombinant harboring phbA and phbB produced 35% PHBHHx with 9 mol% 3HHx in 15 g/L CDW under the same conditions. The obvious differences in terms of the cell growth and PHBHHx production were attributed to the expression of vgb in A. hydrophila 4AK4, which was clearly observed in carbon monoxide difference spectra. The expression of vgb in the recombinant not only improved cell growth and PHBHHx accumulation, but also increased the plasmid stability during cell growth, especially under low dissolved oxygen tension in fermentors. PHBHHx production could be further increased to over 60% of the CDW by the over expression of phaC and phaJ from Aeromonas caviae encoding PHBHHx synthase and (R)-specific enoyl-CoA hydratase, respectively. Over expression of phaC, phaJ and phaP, alone or in various combinations, also increased the 3HHx content of PHBHHx from 14-34%. The above results showed that A. hydrophila was amenable to genetic manipulation, and that these modifications could be exploited to produce compounds with different proper-ties for commercial and research applications.
会议录出版者	WILEY-V C H VERLAG GMBH ; WEINHEIM ; PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY
语种	英语 ; 英语
内容类型	会议论文
源URL	[http://hdl.handle.net/123456789/20002]
专题	清华大学
推荐引用方式 GB/T 7714	Ouyang, SP,Han, J,Qiu, YZ,et al. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) production in recombinant Aeromonas hydrophila 4AK4 harboring phbA, phbB and vgb genes[C]. 见:MACROMOLECULAR SYMPOSIA, 8th World Conference on Biodegradable Polymers and Plastics, Seoul, SOUTH KOREA, Web of Science, INSPEC.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。