One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber

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	One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber
	Fan, Jinxin 1,2; Luo, Jianquan 1,2; Song, Weijie 1; Wan, Yinhua 1,2
刊名	JOURNAL OF MEMBRANE SCIENCE
	2017-04-15
卷号	528 页码:155-162
关键词	alpha(1)-arantitrypsin Cationic polyelectrolyte Polydopamine Anion-exchange membrane adsorber Membrane chromatography
ISSN号	0376-7388
英文摘要	One-step purification of biomolecules from complex feed is challenging with commercial membrane adsorbers since it is difficult to screen and regulate the ligands on the membrane with traditional techniques for removal of various impurities. By readily grafting cationic polyelectrolyte ligands on a polydopamine (PDA)-coated membrane, a membrane adsorber with suitable ligand (750 kDa polyethylenimine, PEI) was selected to achieve one-step purification of arantitrypsin (AAT) from pretreated human plasma fraction W. It was found that ligand structure and molecular weight showed a significant effect on the properties and separation performance of membrane adsorbers. The attached polyelectrolytes increased membrane hydrophilicity but caused an enhancement of filtration resistance, and quaternization of the ligands (PEI and polyallylamine) further improved the hydrophilicity but decreased the charge quantity. By further screening elution salt concentration, flow rate, buffer pH, loading volume as well as molecular weight of PEI ligands, a high activity recovery of 96.6% and a high purity of 94.6% of AAT were obtained by one-step membrane chromatography, which was even better than those by multiple-step method. This could be owing to the special multilayer structure of PEI chains, which could suppress the impurity protein binding on the adsorption sites by exclusion effect. The present work not only provides new insights into the purification of biomolecules from complex streams with polyelectrolytecoupled membrane adsorbers, but also offers a new approach to design the ligands on membrane adsorber for specific application.
WOS标题词	Science & Technology ; Technology ; Physical Sciences
类目[WOS]	Engineering, Chemical ; Polymer Science
研究领域[WOS]	Engineering ; Polymer Science
关键词[WOS]	MIXED MATRIX MEMBRANE ; TRANSFER RADICAL POLYMERIZATION ; ION-EXCHANGE CHROMATOGRAPHY ; HOLLOW-FIBER MEMBRANE ; PROTEIN-BINDING ; HUMAN-PLASMA ; INHIBITOR CONCENTRATE ; FOULING-RESISTANT ; HIGH-CAPACITY ; SEPARATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000395956400016
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/22138]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fan, Jinxin,Luo, Jianquan,Song, Weijie,et al. One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber[J]. JOURNAL OF MEMBRANE SCIENCE,2017,528:155-162.
APA	Fan, Jinxin,Luo, Jianquan,Song, Weijie,&Wan, Yinhua.(2017).One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber.JOURNAL OF MEMBRANE SCIENCE,528,155-162.
MLA	Fan, Jinxin,et al."One-step purification of alpha(1)-antitrypsin by regulating polyelectrolyte ligands on mussel-inspired membrane adsorber".JOURNAL OF MEMBRANE SCIENCE 528(2017):155-162.