YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 特种无机涂层重点实验室 > 期刊论文

	YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure
	Pan, Houhua 1,2,3; Xie, Youtao 1; Zhang, Zequan 1; Li, Kai 1; Hu, Dandan 1; Zheng, Xuebin 1; Fan, Qirning 2; Tang, Tingting 2
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2017-04-01
卷号	152 页码:344-353
关键词	Hierarchical structure BMSC Differentiation YAP Mechanotransduction
英文摘要	Hierarchical structure mimicking the natural bone microenvironment has been considered as a promising platform to regulate cell functions. We have previously fabricated hierarchical macropore/nanowire structure and evidence has shown that it can better manipulate the cytoskeleton status and osteogenic performance of osteoblasts. However, how cues of hierarchical structure are translated and ultimately linked to BMSC lineage commitment have still remained elusive, which hinders the accurate knowledge and further development of the hierarchical structure. In this study, bone marrow-derived mesenchymal stem cells (BMSCs) fate on hierarchical structure was investigated as well as the detailed mechanisms. It was shown that well-developed cytoskeleton and focal adhesion were observed for BMSCs on hierarchical structure, which was accompanied by enhanced osteogenic and depressed adipogenic potential. Evidence of increased YAP activity and nuclear translocation were exhibited on hierarchical structure and YAP knockdown inhibited osteogenic differentiation and promoted adipogenic differentiation induced by hierarchical structure. Further remove of cytoskeleton tension inhibited YAP function, which confirmed the key role of YAP-mediated mechanotransduction in the BMSC differentiation. These results together provide information of the stem cell fate commitment on hierarchical structure and a promising approach to design advanced biomaterials by focusing on specific mechanotransduction process. (C) 2017 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences ; Technology
类目[WOS]	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
研究领域[WOS]	Biophysics ; Chemistry ; Materials Science
关键词[WOS]	STEM-CELL DIFFERENTIATION ; HIPPO PATHWAY ; YAP/TAZ ; SURFACE ; TAZ ; TOPOGRAPHY ; MECHANISM ; ADHESION ; ARRAYS ; FATE
收录类别	SCI
语种	英语
WOS记录号	WOS:000398014100041
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/23766]
专题	上海硅酸盐研究所_特种无机涂层重点实验室_期刊论文
作者单位	1.Chinese Acad Sci, Shanghai Inst Ceram, Key Lab Inorgan Coating Mat, 1295 Dingxi Rd, Shanghai 200050, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai Ninth Peoples Hosp, Shanghai Key Lab Orthopaed Implants,Dept Orthopae, Shanghai 200011, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Pan, Houhua,Xie, Youtao,Zhang, Zequan,et al. YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2017,152:344-353.
APA	Pan, Houhua.,Xie, Youtao.,Zhang, Zequan.,Li, Kai.,Hu, Dandan.,...&Tang, Tingting.(2017).YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure.COLLOIDS AND SURFACES B-BIOINTERFACES,152,344-353.
MLA	Pan, Houhua,et al."YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure".COLLOIDS AND SURFACES B-BIOINTERFACES 152(2017):344-353.