Gut Microbiota and Metabolic Phenotype of Portunus trituberculatus

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Gut Microbiota and Metabolic Phenotype of Portunus trituberculatus
	Zeng Ting-Lan 1; Ye Yang-Fang 1,2; Mu Chang-Kao 1; Wang Kai 1; Li Rong-Hua 1; Wang Chun-Lin 1
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2016-12-01
卷号	44 期号:12 页码:1867-1873
关键词	Portunus trituberculatus Gut microbiota High-throughput sequencing Metabolic phenotype Nuclear magnetic resonance
英文摘要	16S rRNA gene high. throughput sequencing and nuclear magnetic resonance (NMR). based metabolomics techniques were established to understand the composition of gut microbiota and metabolites of Portunus trituberculatus. Swimming crab gut samples were used for the extraction of bacterial genomic DNA, followed by the amplification of hypervariable domain V3-V4 of 16 S rRNA gene. The obtained PCR products were used for analysis on an Illumina MiSeq platform. The bacterial phylotypes were then clustered and assigned. The results showed that swimming crab gut was dominated by proteobacteria, bacteroidetes, fusobacteria, tenericutes, and acidobacteria at the phylum level. At the genus level, Photobacterium, Paludibacter, and Propionigenium were enriched in it. Crab intestinal samples were extracted with CH3OH/H2O (2:1, V/V) solution by shaking with a tissuelyser. After the removal of methanol, the resultant supernatants were lyophilized. Each of lyophilized extract was dissolved into Na+/K+ phosphate buffer and then centrifuged at high speed for NMR analysis. A standard noesypr1D was used to acquire H-1 NMR spectra. The 90 degrees pulse length was adjusted to approximately 10 mu s. The recycle delay and mixing time were set to 2 s and 100 ms in sequence. The spectral width was set to 20 ppm. Sixty. four transients were collected into 32 k data points for each spectrum. A range of two. dimensional NMR spectra were acquired for the resonance assignment. The results showed that swimming crab gut metabolome comprised 30 metabolites including some amino acids, organic acids, and amines. In summary, this study provided a method for the systematic analysis of the compositions of gut bacterial community and metabolites of swimming crab.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	CRAB SCYLLA-PARAMAMOSAIN ; CHINESE MITTEN CRAB ; SP NOV. ; HEALTH ; FISH ; SENSITIVITY ; DIVERSITY ; SEQUENCES ; BACTERIA ; DAMSELAE
收录类别	SCI
语种	英语
WOS记录号	WOS:000393290300011
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/10029]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Zeng Ting-Lan,Ye Yang-Fang,Mu Chang-Kao,et al. Gut Microbiota and Metabolic Phenotype of Portunus trituberculatus[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2016,44(12):1867-1873.
APA	Zeng Ting-Lan,Ye Yang-Fang,Mu Chang-Kao,Wang Kai,Li Rong-Hua,&Wang Chun-Lin.(2016).Gut Microbiota and Metabolic Phenotype of Portunus trituberculatus.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,44(12),1867-1873.
MLA	Zeng Ting-Lan,et al."Gut Microbiota and Metabolic Phenotype of Portunus trituberculatus".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 44.12(2016):1867-1873.