Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44(+) fragments

	Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44(+) fragments
	Chang, Peng-Yu 1,2; Jin, Xing 3; Jiang, Yi-Yao 4; Wang, Li-Xian 5; Liu, Yong-Jun 6; Wang, Jin 2
刊名	CELL AND TISSUE RESEARCH
	2016-05-01
卷号	364 期号:2 页码:331-344
关键词	Mesenchymal stem cell CD44 Radiation-induced death Organoids Crypt domains
英文摘要	Intestinal stem cells are primitive cells found within the intestinal epithelium that play a central role in maintaining epithelial homeostasis through self-renewal and commitment into functional epithelial cells. Several markers are available to identify intestinal stem cells, such as Lgr5, CD24 and EphB2, which can be used to sort intestinal stem cells from mammalian gut. Here, we identify and isolate intestinal stem cells from C57BL/6 mice by using a cell surface antigen, CD44. In vitro, some CD44(+) crypt cells are capable of forming "villus-crypt"aEuro"like structures (organoids). A subset strongly positive for CD44 expresses high levels of intestinal stem-cell-related genes, including Lgr5, Bmi1, Hopx, Lrig1, Ascl2, Smoc2 and Rnf43. Cells from this subset are more capable of developing into organoids in vitro, compared with the subset weakly positive for CD44. However, the organoids are sensitive to ionizing irradiation. We investigate the specific roles of mesenchymal stem cells in protecting organoids against radiation-induced crypt death. When co-cultured with mesenchymal stem cells, the crypt domains of irradiated organoids possess more proliferative cells and fewer apoptotic cells than those not co-cultured with mesenchymal stem cells. Cd44v6 continues to be expressed in the crypt domains of irradiated organoids co-cultured with mesenchymal stem cells. Our results indicate specific roles of mesenchymal stem cells in delaying radiation-induced crypt death in vitro.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Cell Biology
研究领域[WOS]	Cell Biology
关键词[WOS]	IN-VITRO ; PROGENITOR CELLS ; COLON-CANCER ; MARKER ; MICE ; LGR5 ; EPITHELIUM ; ACTIVATION ; POPULATION ; ORGANOIDS
收录类别	SCI
语种	英语
WOS记录号	WOS:000374973300010
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2257]
专题	天津工业生物技术研究所_总体研究部_期刊论文
作者单位	1.Jilin Univ, Dept Radiat Oncol, Bethune Hosp 1, Changchun 130021, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130000, Peoples R China 3.Ever Union Biotechol, Tianjin 300162, Peoples R China 4.TEDA Int Cardiovasc Hosp, Dept Cardiac Surg, Tianjin 300000, Peoples R China 5.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300300, Peoples R China 6.Alliancells Biosci, Tianjin 300300, Peoples R China
推荐引用方式 GB/T 7714	Chang, Peng-Yu,Jin, Xing,Jiang, Yi-Yao,et al. Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44(+) fragments[J]. CELL AND TISSUE RESEARCH,2016,364(2):331-344.
APA	Chang, Peng-Yu,Jin, Xing,Jiang, Yi-Yao,Wang, Li-Xian,Liu, Yong-Jun,&Wang, Jin.(2016).Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44(+) fragments.CELL AND TISSUE RESEARCH,364(2),331-344.
MLA	Chang, Peng-Yu,et al."Mensenchymal stem cells can delay radiation-induced crypt death: impact on intestinal CD44(+) fragments".CELL AND TISSUE RESEARCH 364.2(2016):331-344.