A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320

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	A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320
	Dong, Huina 1,2; Li, Sha 1; Fang, Huan 1,2; Xia, Miaomiao 1,2; Zheng, Ping 1,2; Zhang, Dawei 1,2; Sun, Jibin 1,2
刊名	BIOPROCESS AND BIOSYSTEMS ENGINEERING
	2016-10-01
卷号	39 期号:10 页码:1527-1537
关键词	Vitamin B-12 Sinorhizobium meliloti Cobalamin Whole genome sequence Response surface methodology
英文摘要	Vitamin B-12 (Cobalamin, VB12) has several physiological functions and is widely used in pharmaceutical and food industries. A new unicellular species was extracted from China farmland, and the strain could produce VB12 which was identified by HPLC and HPLC-MS/MS. 16S rDNA analysis reveals this strain belongs to the species Sinorhizobium meliloti and we named it S. meliloti 320. Its whole genome information indicates that this strain has a complete VB12 synthetic pathway, which paves the way for further metabolic engineering studies. The optimal carbon and nitrogen sources are sucrose and corn steep liquor (CSL) plus peptone. The optimal combination of sucrose and CSL was obtained by response surface methodology as they are the most suitable carbon and nitrogen sources, respectively. This strain could produce 140 +/- 4.2 mg L-1 vitamin B-12 after incubating for 7 days in the optimal medium.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	PSEUDOMONAS-DENITRIFICANS ; MICROBIAL-PRODUCTION ; B-12 ; REMOVAL ; LIGAND ; ACID ; DNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000382117800004
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2923]
专题	天津工业生物技术研究所_系统与合成生物技术实验室孙际宾_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Dong, Huina,Li, Sha,Fang, Huan,et al. A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2016,39(10):1527-1537.
APA	Dong, Huina.,Li, Sha.,Fang, Huan.,Xia, Miaomiao.,Zheng, Ping.,...&Sun, Jibin.(2016).A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320.BIOPROCESS AND BIOSYSTEMS ENGINEERING,39(10),1527-1537.
MLA	Dong, Huina,et al."A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320".BIOPROCESS AND BIOSYSTEMS ENGINEERING 39.10(2016):1527-1537.