De novo biosynthesis of Gastrodin in Escherichia coli

CORC > 天津工业生物技术研究所 > 天然化合物合成生物学实验室刘涛 > 期刊论文

	De novo biosynthesis of Gastrodin in Escherichia coli
	Bai, Yanfen 1,2,3; Yin, Hua 1,2; Bi, Huiping 1,2; Zhuang, Yibin 1,2; Liu, Tao 1,2; Ma, Yanhe 1
刊名	METABOLIC ENGINEERING
	2016-05-01
卷号	35 页码:138-147
关键词	Gastrodin Chorismate Metabolic engineering Glycosyltransferase Directed evolution Escherichia coli
英文摘要	Gastrodin, a phenolic glycoside, is the key ingredient of Gastrodia elata, a notable herbal plant that has been used to treat various conditions in oriental countries for centuries. Gastrodin is extensively used clinically for its sedative, hypnotic, anticonvulsive and neuroprotective properties in China. Gastrodin is usually produced by plant extraction or chemical synthesis, which has many disadvantages. Herein, we report unprecedented microbial synthesis of gastrodin via an artificial pathway. A Nocardia carboxylic acid reductase, endogenous alcohol dehydrogenases and a Rhodiola glycosyltransferase UGT73B6 transformed 4-hydroxybenzoic acid, an intermediate of ubiquinone biosynthesis, into gastrodin in Escherichia coli. Pathway genes were overexpressed to enhance metabolic flux toward precursor 4-hydroxybenzyl alcohol. Furthermore, the catalytic properties of the UGT73B6 toward phenolic alcohols were improved through directed evolution. The finally engineered strain produced 545 mg l(-1) gastrodin in 48 h. This work creates a new route to produce gastrodin, instead of plant extractions and chemical synthesis. (C) 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	RADICAL SCAVENGING ACTIVITIES ; PHENOLIC CONSTITUENTS ; SUBSTRATE-SPECIFICITY ; DIRECTED EVOLUTION ; VANILLA-PLANIFOLIA ; ELATA ; ACID ; GLYCOSYLTRANSFERASE ; YIELD ; PRODUCTS
收录类别	SCI
语种	英语
WOS记录号	WOS:000373167900016
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2235]
专题	天津工业生物技术研究所_天然化合物合成生物学实验室刘涛_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Bai, Yanfen,Yin, Hua,Bi, Huiping,et al. De novo biosynthesis of Gastrodin in Escherichia coli[J]. METABOLIC ENGINEERING,2016,35:138-147.
APA	Bai, Yanfen,Yin, Hua,Bi, Huiping,Zhuang, Yibin,Liu, Tao,&Ma, Yanhe.(2016).De novo biosynthesis of Gastrodin in Escherichia coli.METABOLIC ENGINEERING,35,138-147.
MLA	Bai, Yanfen,et al."De novo biosynthesis of Gastrodin in Escherichia coli".METABOLIC ENGINEERING 35(2016):138-147.