Efficiency Analysis and Mechanism Insight of that Whole-Cell Biocatalytic Production of Melibiose from Raffinose with Saccharomyces cerevisiae

CORC > 天津工业生物技术研究所 > 功能糖与天然活性物质研究组孙媛霞 > 期刊论文

	Efficiency Analysis and Mechanism Insight of that Whole-Cell Biocatalytic Production of Melibiose from Raffinose with Saccharomyces cerevisiae
	Zhou, Yingbiao 1,2; Zhu, Yueming 2; Dai, Longhai 2; Men, Yan 2; Wu, Jinhai 3; Zhang, Juankun 1; Sun, Yuanxia 2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2017
卷号	181 期号:1 页码:407-423
关键词	Whole-cell biocatalysis Saccharomyces cerevisiae Characteristics analysis Melibiose Raffinose
英文摘要	Melibiose is widely used as a functional carbohydrate. Whole-cell biocatalytic production of melibiose from raffinose could reduce its cost. However, characteristics of strains for whole-cell biocatalysis and mechanism of such process are unclear. We compared three different Saccharomyces cerevisiae strains (liquor, wine, and baker's yeasts) in terms of concentration variations of substrate (raffinose), target product (melibiose), and by-products (fructose and galactose) in whole-cell biocatalysis process. Distinct difference was observed in whole-cell catalytic efficiency among three strains. Furthermore, activities of key enzymes (invertase, alpha-galactosidase, and fructose transporter) involved in process and expression levels of their coding genes (suc2, mel1, and fsy1) were investigated. Conservation of key genes in S. cerevisiae strains was also evaluated. Results show that whole-cell catalytic efficiency of S. cerevisiae in the raffinose substrate was closely related to activity of key enzymes and expression of their coding genes. Finally, we summarized characteristics of producing strain that offered advantages, as well as contributions of key genes to excellent strains. Furthermore, we presented a dynamic mechanism model to achieve some mechanism insight for this whole-cell biocatalytic process. This pioneering study should contribute to improvement of whole-cell biocatalytic production of melibiose from raffinose.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	SIGNAL-SEQUENCE TRAP ; ALPHA-GALACTOSIDASE ; FRUCTOSE/H+ SYMPORTER ; SECRETED PROTEINS ; YEAST-STRAIN ; INVERTASE ; GENE ; FSY1 ; CLONING
收录类别	SCI
语种	英语
WOS记录号	WOS:000392488300026
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2274]
专题	天津工业生物技术研究所_功能糖与天然活性物质研究组孙媛霞 _期刊论文
作者单位	1.Tianjin Univ Sci & Technol, Key Lab Ind Fermentat Microbiol, Minist Educ, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Natl Engn Lab Ind Enzymes, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Shanxi Normal Univ, Sch Food Sci, Linfen 041000, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yingbiao,Zhu, Yueming,Dai, Longhai,et al. Efficiency Analysis and Mechanism Insight of that Whole-Cell Biocatalytic Production of Melibiose from Raffinose with Saccharomyces cerevisiae[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2017,181(1):407-423.
APA	Zhou, Yingbiao.,Zhu, Yueming.,Dai, Longhai.,Men, Yan.,Wu, Jinhai.,...&Sun, Yuanxia.(2017).Efficiency Analysis and Mechanism Insight of that Whole-Cell Biocatalytic Production of Melibiose from Raffinose with Saccharomyces cerevisiae.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,181(1),407-423.
MLA	Zhou, Yingbiao,et al."Efficiency Analysis and Mechanism Insight of that Whole-Cell Biocatalytic Production of Melibiose from Raffinose with Saccharomyces cerevisiae".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 181.1(2017):407-423.