Effective multi-step functional biotransformations of steroids by a newly isolated Fusarium oxysporum SC1301

CORC > 天津工业生物技术研究所 > 发酵与生物化工工程李正华 > 期刊论文

	Effective multi-step functional biotransformations of steroids by a newly isolated Fusarium oxysporum SC1301
	Zhang, Hongliu 1,2; Ren, Jie 2; Wang, Yu 2; Sheng, Cuo 1,2; Wu, Qiaqing 2; Diao, Aipo 1; Zhu, Dunming 2
刊名	TETRAHEDRON
	2013-01-07
卷号	69 期号:1 页码:184-189
关键词	Biotransformation Steroid Testolactone Fusarium oxysporum Baeyer-Villiger oxidation
英文摘要	A fungus Fusarium oxysporum SC1301 was isolated from soil samples, which could transform androst-4-ene-3,17-dione, androst-1,4-diene-3,17-dione, dehydroepiandrosterone, progesterone, testosterone, and pregnenolone to give testolactone in 76-98% yields. Especially, for progesterone and pregnenolone, multi-step functional transformations, including oxygenative esterification of 20-ketosteroids, hydrolysis of ester, oxidation of C-17 OH group, oxygenative lactonization of 17-ketosteroids, 1-dehydrogenation, oxidation of C-3 OH group and Delta(5 -> 4) C=C bond migration, could proceed effectively to yield testolactone as a single product. This is the first example that a E oxysporum species shows catalytic capability of ring-D lactonization. The results are remarkably distinguished from those previously reported for the closely-related fungus F. oxysporum var. cubense, which predominantly mediates the hydroxylations at different positions of steroids. In addition, E oxysporum SC1301 may serve as a valuable biocatalyst for the production of testolactone. (C) 2012 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Organic
研究领域[WOS]	Chemistry
关键词[WOS]	BAEYER-VILLIGER OXIDATION ; ASPERGILLUS-TAMARII KITA ; AROMATASE INHIBITORS ; TRANSFORMATION ; LACTONIZATION ; HYDROXYLATION ; DEGRADATION ; PATHWAY
收录类别	SCI
语种	英语
WOS记录号	WOS:000313386100023
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2263]
专题	天津工业生物技术研究所_发酵与生物化工工程李正华_期刊论文
作者单位	1.Tianjin Univ Sci & Technol, Coll Biotechnol, Econ & Technol Dev Area, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hongliu,Ren, Jie,Wang, Yu,et al. Effective multi-step functional biotransformations of steroids by a newly isolated Fusarium oxysporum SC1301[J]. TETRAHEDRON,2013,69(1):184-189.
APA	Zhang, Hongliu.,Ren, Jie.,Wang, Yu.,Sheng, Cuo.,Wu, Qiaqing.,...&Zhu, Dunming.(2013).Effective multi-step functional biotransformations of steroids by a newly isolated Fusarium oxysporum SC1301.TETRAHEDRON,69(1),184-189.
MLA	Zhang, Hongliu,et al."Effective multi-step functional biotransformations of steroids by a newly isolated Fusarium oxysporum SC1301".TETRAHEDRON 69.1(2013):184-189.