Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity

CORC > 天津工业生物技术研究所 > 蛋白质工程与疫苗实验室高福 > 期刊论文

	Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity
	Turnbull, Matthew L.1; Wise, Helen M.1,8; Nicol, Marlynne Q.1; Smith, Nikki 1; Dunfee, Rebecca L.2; Beard, Philippa M.1; Jagger, Brett W.2,3; Ligertwood, Yvonne 1; Hardisty, Gareth R.1; Xiao, Haixia 4,9
刊名	JOURNAL OF VIROLOGY
	2016-10-01
卷号	90 期号:20 页码:9263-9284
英文摘要	Two alleles of segment 8 (NS) circulate in nonchiropteran influenza A viruses. The A allele is found in avian and mammalian viruses, but the B allele is viewed as being almost exclusively found in avian viruses. This might reflect the fact that one or both of its encoded proteins (NS1 and NEP) are maladapted for replication in mammalian hosts. To test this, a number of clade A and B avian virus-derived NS segments were introduced into human H1N1 and H3N2 viruses. In no case was the peak virus titer substantially reduced following infection of various mammalian cell types. Exemplar reassortant viruses also replicated to similar titers in mice, although mice infected with viruses with the avian virus-derived segment 8s had reduced weight loss compared to that achieved in mice infected with the A/Puerto Rico/8/1934 (H1N1) parent. In vitro, the viruses coped similarly with type I interferons. Temporal proteomics analysis of cellular responses to infection showed that the avian virus-derived NS segments provoked lower levels of expression of interferon-stimulated genes in cells than wild type-derived NS segments. Thus, neither the A nor the B allele of avian virus-derived NS segments necessarily attenuates virus replication in a mammalian host, although the alleles can attenuate disease. Phylogenetic analyses identified 32 independent incursions of an avian virus-derived A allele into mammals, whereas 6 introductions of a B allele were identified. However, A-allele isolates from birds outnumbered B-allele isolates, and the relative rates of Aves-to-Mammalia transmission were not significantly different. We conclude that while the introduction of an avian virus segment 8 into mammals is a relatively rare event, the dogma of the B allele being especially restricted is misleading, with implications in the assessment of the pandemic potential of avian influenza viruses.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Virology
研究领域[WOS]	Virology
关键词[WOS]	COMPLETE GENOME SEQUENCE ; NS1 PROTEIN ; NUCLEAR EXPORT ; GENETIC-CHARACTERIZATION ; NONSTRUCTURAL PROTEIN-1 ; PLASMA-MEMBRANE ; BETA-INTERFERON ; SOUTHERN CHINA ; MESSENGER-RNAS ; AMINO-ACID
收录类别	SCI
语种	英语
WOS记录号	WOS:000384574900041
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2939]
专题	天津工业生物技术研究所_蛋白质工程与疫苗实验室高福_期刊论文
作者单位	1.Univ Edinburgh, Div Infect & Immun, Roslin Inst, Edinburgh, Midlothian, Scotland 2.NIAID, Viral Pathogenesis & Evolut Sect, Infect Dis Lab, Div Intramural Res, 9000 Rockville Pike, Bethesda, MD 20892 USA 3.Univ Cambridge, Div Virol, Dept Pathol, Cambridge, England 4.Francis Crick Inst, Mill Hill Lab, Mill Hill, London, England 5.Univ Glasgow, Ctr Virus Res, Glasgow, Lanark, Scotland 6.Harvard Med Sch, Dept Cell Biol, Boston, MA USA 7.Univ Cambridge, Cambridge Inst Med Res, Cambridge, England 8.Heriot Watt Univ, Dept Engn & Phys Sci, Edinburgh, Midlothian, Scotland 9.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccines, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Turnbull, Matthew L.,Wise, Helen M.,Nicol, Marlynne Q.,et al. Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity[J]. JOURNAL OF VIROLOGY,2016,90(20):9263-9284.
APA	Turnbull, Matthew L..,Wise, Helen M..,Nicol, Marlynne Q..,Smith, Nikki.,Dunfee, Rebecca L..,...&Digard, Paul.(2016).Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity.JOURNAL OF VIROLOGY,90(20),9263-9284.
MLA	Turnbull, Matthew L.,et al."Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity".JOURNAL OF VIROLOGY 90.20(2016):9263-9284.