Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities | |
Sun, Jiajia1,2; Yendluri, Raghuvara3,4; Liu, Kai1,5; Guo, Ying2; Lvov, Yuri3,4,6; Yan, Xuehai1 | |
刊名 | PHYSICAL CHEMISTRY CHEMICAL PHYSICS |
2017 | |
卷号 | 19期号:1页码:562-567 |
ISSN号 | 1463-9076 |
DOI | 10.1039/c6cp07450b |
文献子类 | Article |
英文摘要 | We have developed a simple and effective strategy to prepare an enzymatic membrane by the admixing of a halloysite clay nanotube-lipase complex and a chitosan solution. The loading of 3 wt% lipase into the lumen of halloysite clay nanotubes was enhanced with an electrostatic interaction. 10 wt% of lipase-nanotubes were dispersed in an aqueous chitosan solution and cast as a coating. The enzymatic chitosan-halloysite membranes displayed increased pH, temperature stability and long-term usability for the catalytic decomposition of lipids: 90% of the initial activity was preserved at 80 degrees C, 70% of the functionality remained at the high pH of 9, and 85% of the activity was sustained after ten continuous cycles of use. The placement of the second antimicrobial enzyme-lysozyme at the outer surface of the clay nanotubes allowed for a synergistic increase in the activity of the composite antibacterial formulation. The lipase/lysozyme-co-immobilized clay nanotube-chitosan membranes are promising in anti-biofouling applications. |
WOS关键词 | Halloysite Nanotubes ; Drug-delivery ; Encapsulation ; Coatings ; Surfaces ; Release ; Chitin ; Films |
WOS研究方向 | Chemistry ; Physics |
语种 | 英语 |
WOS记录号 | WOS:000391725300059 |
资助机构 | National Natural Science Foundation of China(21522307 ; Talent Fund of the Recruitment Program of Global Youth Experts ; Chinese Academy of Sciences (CAS)(QYZDB-SSWISC034) ; Russian Science Foundation(14-19-01045) ; 21473208 ; 91434103) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/21882] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Harbin Univ Sci & Technol, Sch Chem & Environm Engn, Harbin 150080, Peoples R China 3.Louisiana Tech Univ, Inst Micromfg, Ruston, LA 71270 USA 4.Louisiana Tech Univ, Biomed Engn Program, Ruston, LA 71270 USA 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.I Gubkin Russian State Univ Oil & Gas, Moscow 119991, Russia |
推荐引用方式 GB/T 7714 | Sun, Jiajia,Yendluri, Raghuvara,Liu, Kai,et al. Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities[J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS,2017,19(1):562-567. |
APA | Sun, Jiajia,Yendluri, Raghuvara,Liu, Kai,Guo, Ying,Lvov, Yuri,&Yan, Xuehai.(2017).Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities.PHYSICAL CHEMISTRY CHEMICAL PHYSICS,19(1),562-567. |
MLA | Sun, Jiajia,et al."Enzyme-immobilized clay nanotube-chitosan membranes with sustainable biocatalytic activities".PHYSICAL CHEMISTRY CHEMICAL PHYSICS 19.1(2017):562-567. |
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