Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel

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	Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel
	Yang, Dong 1,3; Zhao, Jingjing 1,4; Wang, Xueyan 1,4; Shi, Jiafu 2,3; Zhang, Shaohua 1,4; Jiang, Zhongyi 1,2,4
刊名	BIOCHEMICAL ENGINEERING JOURNAL
	2017-01-15
卷号	117 页码:52-61
关键词	Monolithic biocatalytic systems rGO/FeOOH hydrogel Biomimetic silicification Enhanced stabilities Enzyme immobilization Penicillin G acylase
ISSN号	1369-703X
英文摘要	In this study, we present a green and facile method of utilizing biomimetic silicification to trigger enzyme immobilization on the surface of the rGO/FeOOH hydrogel for constructing stable monolithic biocatalytic systems. In brief, the rGO/FeOOH hydrogel is firstly prepared through metal ion-induced reduction/assembly of graphene oxide (GO) nanosheets, which is then utilized to adsorb cationic polyethyleneimine (PEI). This cationic PEI, as the mineralization-inducing agent, catalyzes the condensation of silicate to form silica (biomimetic silicification) on the rGO surface, where enzyme is simultaneously entrapped. The resultant rGO/FeOOH/silica hydrogel shows an extraordinary three-dimensional (3D) porous structure. The silica content on the rGO surface can be facilely tailored through changing the silica precursor concentration. Combined with monolithic macroscale of the rGO/FeOOH/silica hydrogel, the acquired monolithic biocatalytic systems display easy recyclability and elevated pH/thermal/recycling/storage stabilities during the catalytic production of 6-aminopenicillanic acid (6-APA) in comparison to enzyme in free form and enzyme adsorbed on rGO/FeOOH hydrogel. Notably, the activity can be retained up to 93.3% of its initial activity after 11 reaction cycles for our biocatalytic systems. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	GRAPHENE OXIDE ; RECYCLABLE NANOBIOCATALYST ; HIGH-PERFORMANCE ; NANOPARTICLES ; SILICA ; MINERALIZATION ; COMPOSITES ; AEROGELS ; CARBON ; SHELL
收录类别	SCI
语种	英语
WOS记录号	WOS:000390968200006
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/21868]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Green Chem Technol, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Tianjin Univ, Sch Environm Sci & Engn, Tianjin Engn Ctr Biomass Derived Gas & Oil, Tianjin 300072, Peoples R China 4.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
推荐引用方式 GB/T 7714	Yang, Dong,Zhao, Jingjing,Wang, Xueyan,et al. Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel[J]. BIOCHEMICAL ENGINEERING JOURNAL,2017,117:52-61.
APA	Yang, Dong,Zhao, Jingjing,Wang, Xueyan,Shi, Jiafu,Zhang, Shaohua,&Jiang, Zhongyi.(2017).Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel.BIOCHEMICAL ENGINEERING JOURNAL,117,52-61.
MLA	Yang, Dong,et al."Monolithic biocatalytic systems with enhanced stabilities constructed through biomimetic silicification-induced enzyme immobilization on rGO/FeOOH hydrogel".BIOCHEMICAL ENGINEERING JOURNAL 117(2017):52-61.