Aptamer-initiated on-particle template-independent enzymatic polymerization (aptamer-OTEP) for electrochemical analysis of tumor biomarkers

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Aptamer-initiated on-particle template-independent enzymatic polymerization (aptamer-OTEP) for electrochemical analysis of tumor biomarkers
	Wang, PJ; Wan, Y; Deng, SY; Yang, SL; Su, Y; Fan, CH; Aldalbahi, A; Zuo, XL
刊名	BIOSENSORS & BIOELECTRONICS
	2016
卷号	86 页码:536-541
关键词	Electrochemical aptasensor Nanoprobe Terminal deoxynucleotidyl transferase (TdT) Aptamer-initiated on-particle template-independent enzymatic polymerization (aptamer-OTEP)
ISSN号	0956-5663
通讯作者	Yang, SL (reprint author), Nanjing Univ Sci & Technol, Sch Environm & Biol & Engn, Nanjing 210094, Jiangsu, Peoples R China. ; Wan, Y (reprint author), Nanjing Univ Sci & Technol, Sch Mech Engn, Nanjing 210094, Jiangsu, Peoples R China. ; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai 201800, Peoples R China. ; Zuo, XL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai 201800, Peoples R China.
英文摘要	Herein, an aptamer-initiated on-particle template-independent enzymatic polymerization. (aptamer-OTEP) strategy for electrochemical aptasensor (E-aptasensor) is developed for analysis of cancer biomarker carcino-embryonic antigen (CEA). A pair of DNA aptamers is employed which can be specifically bond with CEA simultaneously. One of the aptamer is thiolated at 3'-terminal and immobilized onto the gold electrode as a capture probe, while the other one has a thiol group at its 5'-terminal and is modified onto the gold nanoparticles surface to form a nanoprobe. In the present of target, the two aptamers can "sandwich" the target, thus the nanoprobe is attached to the electrode. Then terminal deoxynucleotidyl transferase (TdT) is employed to catalyze the incorporation of biotin labeled dNTPs into the 3'-OH terminals of the DNA aptamer on the nanoprobe. The as-generated long DNA oligo tentacles allow specific binding of numerous avidin modified horseradish peroxidase (Av-HRP), resulting in tens of thousands of HRP catalyzed reduction of hydrogen peroxide and sharply increasing electrochemical signals. Taking advantage of the enzyme based nucleic acid amplification and nanoprobe, this strategy is demonstrated to possess the outstanding amplification efficiency. (C) 2016 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000384853300075
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/26432]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Wang, PJ,Wan, Y,Deng, SY,et al. Aptamer-initiated on-particle template-independent enzymatic polymerization (aptamer-OTEP) for electrochemical analysis of tumor biomarkers[J]. BIOSENSORS & BIOELECTRONICS,2016,86:536-541.
APA	Wang, PJ.,Wan, Y.,Deng, SY.,Yang, SL.,Su, Y.,...&Zuo, XL.(2016).Aptamer-initiated on-particle template-independent enzymatic polymerization (aptamer-OTEP) for electrochemical analysis of tumor biomarkers.BIOSENSORS & BIOELECTRONICS,86,536-541.
MLA	Wang, PJ,et al."Aptamer-initiated on-particle template-independent enzymatic polymerization (aptamer-OTEP) for electrochemical analysis of tumor biomarkers".BIOSENSORS & BIOELECTRONICS 86(2016):536-541.