In Vitro and in Vivo Evaluation of Silicate-Coated Polyetheretherketone Fabricated by Electron Beam Evaporation | |
Wen, Jin1,2; Lu, Tao2,3; Wang, Xiao1,2; Xu, Lianyi1,2; Wu, Qianju1,2; Pan, Hongya2; Wang, Donghui3; Liu, Xuanyong3; Jiang, Xinquan1,2 | |
刊名 | ACS APPLIED MATERIALS & INTERFACES |
2016-06-01 | |
卷号 | 8期号:21页码:13197-13206 |
关键词 | PEEK silicate electron beam evaporation stem cells osseointegration ovariectomy |
英文摘要 | Intrinsic bioinertness severely hampers the application of polyetheretherketone (PEEK), although in the, field of dentistry it is considered to be an ideal titanium substitute implanting material. In this study, a bioactive silicate coating, was successfully introduced onto PEEK surface by using electron beam evaporation (EBE) technology to improve its bioactivity and osseointegration of PEEK. Through controlling the duration of EBE, the incorporated amounts of silicon (Si) could be exquisitely adjusted to obtain proper biofunctionality, as assessed by cell adhesion, proliferation, osteogenic gene expression, and protein detection. In vivo, the samples were then tested in a femur implantation model to assay osseointegration effects in ovariectomized (OVX) rats. Remarkable enhancement of adhesion, spreading, osteogenesis, and differentiation of bone marrow stem cells (rBMSCs-OVX) were noted on silicate-coated samples. In particular, the group that was processed for 5 min with EBE (EBB-5 min) showed the most improvements in ALP activity and osteogenic-related gene expression compared to the remaining groups. Better osseointegration of the group that was processed for 8 min with EBE (EBE-8 min) was observed in vivo, as indicated by micro-CT test, fluorescent labeling, and histological and histomorphometric analyses. Collectively, the outcomes of the above experiments demonstrate that the present work is a meaningful attempt to promote osseointegration under osteoporotic conditions with only Si element incorporated to PEEK Surface by the application of EBE technique. To the best of our knowledge, this work is the first demonstration of tuning the surface properties of PEEK via the adoption of an EBE-fabricated silicate coating to address an osteoporotic problem both in vitro and in vivo. |
WOS标题词 | Science & Technology ; Technology |
类目[WOS] | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
研究领域[WOS] | Science & Technology - Other Topics ; Materials Science |
关键词[WOS] | TENSION-TENSION FATIGUE ; SUBSTITUTED HYDROXYAPATITE ; OSTEOGENIC DIFFERENTIATION ; TRICALCIUM PHOSPHATE ; BONE-FORMATION ; PEEK ; TITANIUM ; BIOACTIVITY ; IMPLANTS ; SURFACE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000377150400004 |
内容类型 | 期刊论文 |
源URL | [http://ir.sic.ac.cn/handle/331005/22981] |
专题 | 上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文 |
作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Prosthodont, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9,Shanghai Key Lab Stomatol, Ora1 Bioengn Lab,Shanghai Res Inst Stomatol, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China |
推荐引用方式 GB/T 7714 | Wen, Jin,Lu, Tao,Wang, Xiao,et al. In Vitro and in Vivo Evaluation of Silicate-Coated Polyetheretherketone Fabricated by Electron Beam Evaporation[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(21):13197-13206. |
APA | Wen, Jin.,Lu, Tao.,Wang, Xiao.,Xu, Lianyi.,Wu, Qianju.,...&Jiang, Xinquan.(2016).In Vitro and in Vivo Evaluation of Silicate-Coated Polyetheretherketone Fabricated by Electron Beam Evaporation.ACS APPLIED MATERIALS & INTERFACES,8(21),13197-13206. |
MLA | Wen, Jin,et al."In Vitro and in Vivo Evaluation of Silicate-Coated Polyetheretherketone Fabricated by Electron Beam Evaporation".ACS APPLIED MATERIALS & INTERFACES 8.21(2016):13197-13206. |
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