Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 高性能陶瓷和超微结构国家重点实验室 > 期刊论文

	Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging
	Chen, Fang 1,2; Ma, Ming 3; Wang, Junxin 1; Wang, Fang 4; Chern, Shi-Xiong 3; Zhao, Eric Ruike 1; Jhunjhunwala, Anamik 5; Darmadi, Sean 1; Chen, Hangrong 3; Jokerst, Jesse V.1,2
刊名	NANOSCALE
	2017
卷号	9 期号:1 页码:402-411
英文摘要	Ultrasound is critical in many areas of medicine including obstetrics, oncology, and cardiology with emerging applications in regenerative medicine. However, one critical limitation of ultrasound is the low contrast of target tissue over background. Here, we describe a novel cup-shaped silica nanoparticle that is reminiscent of exosomes and that has significant ultrasound impedance mismatch for labelling stem cells for regenerative medicine imaging. These exosome-like silica nanoparticles (ELS) were created through emulsion templating and the silica precursors bis(triethoxysilyl) ethane (BTSE) and bis(3-tri-methoxysilyl-propyl) amine (TSPA). We found that 40% TSPA resulted in the exosome like-morphology and a positive charge suitable for labelling mesenchymal stem cells. We then compared this novel structure to other silica structures used in ultrasound including Stober silica nanoparticles (SSN), MCM-41 mesoporous silica nanoparticles (MSN), and mesocellular foam silica nanoparticles (MCF) and found that the ELS offered enhanced stem cell signal due to its positive charge to facilitate cell uptake as well as inherently increased echogenicity. The in vivo detection limits were <500 cells with no detectable toxicity at the concentrations used for labelling. This novel structure may eventually find utility in applications beyond imaging requiring an exosome-like shape including drug delivery.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]	TARGETED DRUG-DELIVERY ; IN-VIVO ; MYOCARDIAL-INFARCTION ; REGENERATIVE MEDICINE ; SIZE RANGE ; SURVIVAL ; THERAPY ; CANCER ; NANOMEDICINE ; NANOSPHERES
收录类别	SCI
语种	英语
WOS记录号	WOS:000391739300049
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/23377]
专题	上海硅酸盐研究所_高性能陶瓷和超微结构国家重点实验室_期刊论文
作者单位	1.Univ Calif San Diego, Dept NanoEngn, 9500 Gilman Dr, La Jolla, CA 92093 USA 2.Univ Calif San Diego, Mat Sci & Engn Program, 9500 Gilman Dr, La Jolla, CA 92093 USA 3.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 4.Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China 5.Univ Calif San Diego, Dept Bioengn, 9500 Gilman Dr, La Jolla, CA 92093 USA
推荐引用方式 GB/T 7714	Chen, Fang,Ma, Ming,Wang, Junxin,et al. Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging[J]. NANOSCALE,2017,9(1):402-411.
APA	Chen, Fang.,Ma, Ming.,Wang, Junxin.,Wang, Fang.,Chern, Shi-Xiong.,...&Jokerst, Jesse V..(2017).Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging.NANOSCALE,9(1),402-411.
MLA	Chen, Fang,et al."Exosome-like silica nanoparticles: a novel ultrasound contrast agent for stem cell imaging".NANOSCALE 9.1(2017):402-411.