Evaluating the relationship between cell viability and volatile organic compound production following DMSO treatment of cultured human cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 大气环境科学实验室

	Evaluating the relationship between cell viability and volatile organic compound production following DMSO treatment of cultured human cells
	Li, Zhen; Shu, Jinian; Yang, Bo; Xu, Ce; Zou, Yao; Sun, Wanqi
刊名	PHARMAZIE
	2016-12
卷号	71 期号:12 页码:727-732
英文摘要	Methylsulfinylmethane (dimethyl sulfoxide; DMSO) is widely used in clinical treatment and bioresearch. Moreover, there is bioconversion between methylsulfanylmethane (dimethyl sulfide; DMS), DMSO, and methylsulfonylmethane (DMSO2) in mammalian metabolism. Due to the real-time detection limits for volatile compounds, most research has focused on DMSO2 as a stable byproduct of DMSO. Therefore, details about the production of DMS as a byproduct of DMSO metabolism remain to be elucidated. Here, we report the characterization of trace-level volatile organic compounds (VOCs) produced following DMSO treatment of cultured human cells using an ultrasensitive vacuum ultraviolet photoionization mass spectrometer (VUV-PIMS). Using this approach, 24 h after DMSO treatment we detected 16.9 and 21 parts per billion by volume (ppbv) DMS in the atmosphere above the cells (headspace) within HeLa and 293T tissue culture flasks, respectively. When simultaneously exposed to 50 nM paclitaxel (PTX), 17.6 and 22.3 ppbv DMS were detected in the headspace of HeLa and 293T culture flasks, respectively. Nevertheless, at doses of PTX more or less than 50 nM, the detectable levels of DMS were reduced to as low as 8.4 ppbv. Our experimental results demonstrate that by co-administering 5 to 10 nM PTX with DMSO, it is possible to moderate the production of DMS considerably. However, at higher doses of PTX, increased apoptosis was observed that likely contributed to higher DMS production by cells.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/35705]
专题	生态环境研究中心_大气环境科学实验室
推荐引用方式 GB/T 7714	Li, Zhen,Shu, Jinian,Yang, Bo,et al. Evaluating the relationship between cell viability and volatile organic compound production following DMSO treatment of cultured human cells[J]. PHARMAZIE,2016,71(12):727-732.
APA	Li, Zhen,Shu, Jinian,Yang, Bo,Xu, Ce,Zou, Yao,&Sun, Wanqi.(2016).Evaluating the relationship between cell viability and volatile organic compound production following DMSO treatment of cultured human cells.PHARMAZIE,71(12),727-732.
MLA	Li, Zhen,et al."Evaluating the relationship between cell viability and volatile organic compound production following DMSO treatment of cultured human cells".PHARMAZIE 71.12(2016):727-732.